67名健康儿童供者造血干细胞动员及采集效果分析  

Analysis of effect of hematopoietic stem cell mobilization and collection in 67 healthy pediatric donors

作　　者：吴梅青 陈英华[1] 刘燕晔[1] 李智[1] 刘练金[1] 施玲玲[1] 章忠明[1] Wu Meiqing;Chen Yinghua;Liu Yanye;Li Zhi;Liu Lianjin;Shi Lingling;Zhang Zhongming(Department of Hematology,the First Affiliated Hospital of Guangxi Medical University,Nanning 530021,China)

机构地区：[1]广西医科大学第一附属医院,南宁530021

出　　处：《新医学》2023年第7期487-491,共5页Journal of New Medicine

基　　金：中国博士后科学基金第67批面上项目(2020M673097)。

摘　　要：目的分析健康儿童供者的造血干细胞动员和采集效果。方法67名健康儿童供者纳入研究,根据供/受者体重比值(供/受BWR)分为供/受BWR<1组(24例)、供/受BWR1~2组(29例)和供/受BWR>2组(14例),在各组间比较骨髓和外周血干细胞动员、采集效果、不良反应等情况。结果供/受BWR<1的供者采集的骨髓干细胞单个核细胞数(MNC)明显低于供/受BWR1~2组和供/受BWR>2组(P均<0.001),而3组间骨髓CD34^(+)细胞数比较差异无统计学意义(P>0.05)。供/受BWR>2组的外周血干细胞MNC明显高于供/受BWR<1组和供/受BWR1~2组(P<0.05),且供/受BWR>2组的外周血干细胞CD34^(+)细胞数明显高于供/受BWR<1组(P=0.032)。健康儿童供者动员和采集干细胞过程中不良反应可耐受,所采集的骨髓和外周血干细胞均能满足移植需求,随访其相应受者无植入不良发生。结论健康儿童作为供者进行造血干细胞采集效果良好,采集过程安全可行。尽管低供/受BWR供者采集的干细胞比高供/受BWR供者低,但所采集的干细胞均能满足移植需求。Objective To evaluate the effect of hematopoietic stem cell mobilization and collection in healthy pediatric donors.Methods 67 healthy pediatric donors were included in this study.According to the donor/recipient(D/R)body weight ratio(BWR),they were divided into three groups:D/R BWR<1 group(n=24),D/R BWR 1-2 group(n=29),and D/R BWR>2 group(n=14).The mobilization,collection effect and adverse reactions of bone marrow and peripheral blood stem cells were compared among all groups.Results The quantity of mononuclear cells(MNCs)of bone marrow stem cells collected in the D/R BWR<1 group was significantly lower than those in the D/R BWR 1-2 and D/R BWR>2 group(both P<0.001).There was no significant difference in the quantity of bone marrow CD34^(+)cells among three groups(all P>0.05).The quantity of MNCs of peripheral blood stem cells in the D/R BWR>2 group was significantly higher than those in the D/R BWR<1 and D/R BWR 1-2 group(both P<0.05),and the quantity of CD34^(+)cells of peripheral blood stem cells in the D/R BWR>2 group was significantly higher than that in the D/R BWR<1 group(P=0.032).Adverse reactions were tolerated in all donors during stem cell mobilization and collection.The bone marrow and peripheral blood stem cells collected from pediatric donors could meet the transplantation needs.No poor implantation or implantation failure occurred in the related recipients during follow-up.Conclusions Hematopoietic stem cell collection from healthy pediatric donors is effective and safe.Although the quantity of stem cells collected in low D/R BWR is less compared to that in the high D/R BWR,the collected stem cells can meet the transplantation needs.

关 键 词：儿童供者 造血干细胞动员 造血干细胞采集 安全性 

分 类 号：R457.7[医药卫生—治疗学]