鲍曼不动杆菌DP-2降解邻苯二甲酸酯的广谱性及降解途径的初步研究  

Primary research of broad-spectrum and biodegradation pathways of phthalic acid esters by Acinetobacter baumannii DP-2

作　　者：李成 刘春敬 柳月 李博文 谢建治 LI Cheng;LIU Chunjing;LIU Yue;LI Bowen;XIE Jianzhi(College of Resources and Environmental Science,Hebei Agricultural University,Key Laboratory for Farmland Eco-Environment of Hebei Province,Baoding 071001,China;State Key Laboratory of North China Crop Improvement and Regulation,Baoding 071001,China)

机构地区：[1]河北农业大学资源与环境科学学院,河北省农田生态环境重点实验室,河北保定071001 [2]省部共建华北作物改良与调控国家重点实验室,河北保定071001

出　　处：《林业与生态科学》2023年第3期300-310,共11页Forestry and Ecological Sciences

基　　金：国家自然科学基金项目(51908189);河北省自然科学基金(2022204029)。

摘　　要：微生物降解是修复邻苯二甲酸酯(PAEs)污染土壤的有效途径,本研究以15种邻苯二甲酸酯(PAEs)为目标污染物,研究鲍曼不动杆菌DP-2对直链、侧链及环状取代PAEs的生物降解特性和降解途径。研究结果表明,直链取代PAEs中,随着烷基碳原子数增加,DP-2对PAEs的降解率逐渐升高,其对邻苯二甲酸二丁酯(DBP)降解率最高,为94.52%;对邻苯二甲酸二甲酯(DMP)的降解率最低,为42.30%;侧链取代PAEs中,DP-2对邻苯二甲酸二异丁酯(DIBP)、邻苯二甲酸二(2-乙基己基)酯(DEHP)、邻苯二甲酸二丙烯酯(DAP)、邻苯二甲酸二(2-甲氧基)乙酯(DMEP)的降解率分别为92.33%、52.22%、77.31%和27.75%;环状取代PAEs中,DP-2对邻苯二甲酸丁苄酯(BBP)、邻苯二甲酸二苯酯(DPHP)、邻苯二甲酸二环己酯(DCHP)的降解率分别为89.87%、80.56%和62.91%。在此基础上,以DBP为代表性PAEs,采用GC-MS对DP-2的主要降解产物进行了鉴定,主要由DMP、MBP、PA和苯甲酸衍生物组成,DBP主要通过转酯化和水解2个途径转化为DMP和MBP,进一步转化为PA,继续被降解为BA。通过对不同取代PAEs降解途径分析,进一步证实了菌株DP-2对PAEs的降解途径。本研究结果表明DP-2降解PAEs具有较好的广谱性,在土壤PAEs污染修复领域具有较好的应用前景。Microbial degradation was an effective way to repair soil contaminated with phthalate esters(PAEs).In this study,a total of fifteen phthalic acid esters(PAEs)were selected as target pollutants to investigate the biodegradation characteristics and degradation pathways of different straight-chain,side-chain and cyclic-chain PAEs by strain Acinetobacter baumannii DP-2.The results indicated that the degradation rate of PAEs by DP-2 increased gradually with the increasing of alkyl carbon atom number in straight-chain PAEs.The highest degradation rate appeared in the dibutyl phthalate(DBP)treatment with the value of 94.52%while the lowest value appeared in the dimethyl phthalate(DMP)treatment which was 42.30%.For the studied side-chain PAEs,the degradation rates of diisobutyl phthalate(DIBP),di-(2-ethylhexyl)phthalate(DEHP),diallyl phthalate(DAP)and dimethoxyethyl phthalate(DMEP)of DP-2 were 92.33%,52.22%,77.31%and 27.75%,respectively.For the studied cyclic-chain PAEs,the degradation rates of butyl benzyl phthalate(BBP),diphenyl phthalate(DPHP)and dicyclohexyl phthalate(DCHP)of DP-2 were 89.87%,80.56%and 62.91%,respectively.Taking DBP as the representative PAEs,the intermediate metabolites of DP-2 were identified by GC-MS,which were mainly composed of DMP,MBP,PA and benzoic acid derivatives.DBP was firstly degraded into DMP and MBP via transesterifification and hydrolysis,further converted into PA,continued to be degraded into BA.The degradation process of PAEs by DP-2 was further confirmed by analyzing the degradation pathway of different substituted PAEs.The above results proved DP-2 had quite broad spectrum and had good application potential for soil PAEs remediation.

关 键 词：邻苯二甲酸酯 生物降解 广谱性 降解途径 

分 类 号：X592[环境科学与工程—环境工程]