长茎葡萄蕨藻益藻细菌筛选及性能评价  

Screening and Performance Evaluation of Beneficial Bacteria from Caulerpa lentillifera

作　　者：王思盼 陈柏安 黄永健 林坤 崔建军 刘东超[1,2] WANG Si-pan;CHEN Bai-an;HUANG Yong-jian;LIN Kun;CUI Jian-jun;LIU Dong-chao(Guangdong Ocean University,College of Fisheries,Zhanjiang 524088,China;Engineering Technology Research Center for Algae Breeding and Application of Guangdong Province,Zhanjiang 524025,China)

机构地区：[1]广东海洋大学水产学院,广东湛江524088 [2]广东省藻类养殖及应用工程技术中心,广东湛江524025

出　　处：《广东海洋大学学报》2023年第4期84-92,共9页Journal of Guangdong Ocean University

基　　金：广东省科技计划(2015A020209167)。

摘　　要：【目的】筛选长茎葡萄蕨藻(Caulerpa lentillifera)益藻细菌并评价其促生长性能,验证菌株对藻体的促生长效果。【方法】通过16S rDNA测序和平板涂布法对养殖长茎葡萄蕨藻进行藻际细菌菌群多样性分析、藻际可培养细菌分离鉴定及其促生特性分析;将获得的菌株制成菌悬液,与长茎葡萄蕨藻培养液按照体积比1∶400混合构建藻菌共生功能体,进一步验证菌株对藻体的促生效果。【结果】长茎葡萄蕨藻的附生细菌在门水平上以变形菌门和拟杆菌门为主要优势类群,分别占总菌的46.0%和41.2%;通过平板涂布法分离培养共获得9株细菌菌株,经鉴定属于8个属,分别为Formosa属、假鲁杰氏菌属(Pseudoruegeria)、短杆菌属(Brevibacterium)、深海单胞菌属(Thalassomonas)、Tenacibaculum属、鲁杰氏菌属(Ruggeria),弧菌属(Vibrio)和芽孢杆菌属(Bacillus),其中6株具有溶解有机磷能力的菌株分属于Formosa属、假鲁杰氏菌属、短杆菌属、鲁杰氏菌属和弧菌属,5株具有溶解无机磷能力的菌株分属于Formosa属、短杆菌属、深海单胞菌属和芽孢杆菌属,3株具有固氮能力的菌株分属于假鲁杰氏菌属、Tenacibaculum属和弧菌属,1株具有产吲哚-3-乙酸(IAA)能力和1株可以生产铁载体的菌株依次分属于弧菌属和深海单胞菌属。在藻菌共培养中,菌株Cl-9菌悬液可极显著地促进长茎葡萄蕨藻的生长(P<0.01)。【结论】筛选出一株长茎葡萄蕨藻益藻细菌(Bacillus sp.Cl-9),该菌可以与长茎葡萄蕨藻构建稳定的共生体系,显著提高藻体的生长,具有应用于工厂化养殖的潜力。【Objective】To screen the beneficial bacteria of Caulerpa lentillifera,evaluate its growth-promoting ability,and verify the growth-promoting effect of the strains on the algae.【Method】The study examined the diversity of the bacterial community in the algal sphere of C.lentillifera,the isolation and identification of cultivable bacteria in the phycosphere,and their growth-promoting characteristics by 16S rDNA sequencing and plate coating method.To further demonstrate the strain's ability to promote the growth of C.lentillifera,,the bacterial suspension was prepared and combined with the C.lentillifera culture medium at a volume ratio of 1:400.【Results】The findings revealed that Proteobacteria and Bacteroidetes,accounting for 46.0%and 41.2%of the total bacteria,respectively,were the predominant epiphytic bacteria at the phylum level.Using the plate coating separation method,nine strains were isolated and cultured for further examination.Based on analyses of 16S rDNA gene sequences,these strains were affiliated to the genera:Formosa,Pseudoruegeria,Brevibacterium,Thalassomonas,Tenacibaculum,Ruggeria,Vibrio and Bacillus,respectively.Moreover,among the examined strains,6 strains capable of dissolving organic phosphorus were classified into the Formosa,Pseudoruegeria,Brevibacterium,Ruggeria and Vibrio.5 strains capable of dissolving inorganic phosphorus were classified into the Formosa,Brevibacterium,Thalassomonas and Bacillus.3 strains capable of fixing nitrogen were classified into the Pseudoruegeria,Tenacibaculum and Vibrio.1 strain capable of producing indole-3-acetic acid and 1 strain capable of producing siderophores were classified into the Vibrio and Thalassomonas,successively.The outcomes demonstrated that the suspension of strain Cl-9 aided the growth of C.lentillifera very significantly(P<0.01).【Conclusion】The Bacillus sp.Cl-9 strain had the potential to be used in factory culture because it could create a reliable symbiotic relationship with C.lentillifera and greatly enhance the growth of the

关 键 词：长茎葡萄蕨藻 藻菌共生 藻际菌群 促生菌 

分 类 号：Q939.9[生物学—微生物学]