消毒剂对重组Ⅲ型人源化胶原蛋白促细胞黏附活性的影响  

Effect of disinfectants on cell adhesion activity of recombinant typeⅢhumanized collagen

作　　者：李怡桐 孙艳艳 乔绍俊 侯丽莉 李熳琴 于玉凤 Yitong Li;Yanyan Sun;Shaojun Qiao;Lili Hou;Manqin Li;Yufeng Yu(Haide College,Ocean University of China,Qingdao,Shandong 266100,China;Shanxi Key Laboratory of Functional Proteins,Shanxi Jinbo Bio-Pharmaceutical Co.,Ltd.,Taiyuan,Shanxi 030032,China;College of Basic Medical Sciences,Shanxi Medical University,Taiyuan,Shanxi 030001,China)

机构地区：[1]中国海洋大学海德学院,山东青岛266100 [2]山西锦波生物医药股份有限公司功能蛋白山西省重点实验室,山西太原030032 [3]山西医科大学基础医学院,山西太原030001

出　　处：《日用化学工业（中英文）》2023年第7期789-795,共7页China Surfactant Detergent & Cosmetics

基　　金：山西省重点研发计划资助项目(201903D311008)。

摘　　要：通过CCK8实验探究75%乙醇、碘伏、葡萄糖酸洗必泰、苯扎氯铵、苯扎溴铵对细胞的毒性以及对重组Ⅲ型人源化胶原蛋白(rhColⅢ)促细胞的黏附活性的影响,预测消毒剂的使用在面部填充时对胶原蛋白活性的影响,为临床应用提供指导。结果显示:各消毒剂对NIH/3T3小鼠胚胎成纤维细胞和HFB人皮肤成纤维细胞均表现出细胞毒性,比较结果为:葡萄糖酸洗必泰<苯扎氯铵、苯扎溴铵<75%乙醇、碘伏,该结果也间接提示75%乙醇、碘伏消毒效果较佳;将各消毒剂原液稀释100倍后,其对rhColⅢ促细胞黏附活性影响比较为:葡萄糖酸洗必泰、苯扎氯铵<75%乙醇<碘伏、苯扎溴铵。碘伏和苯扎溴铵工作液直接接触rhColⅢ均显著降低其促细胞黏附活性,碘伏、葡萄糖酸洗必泰、苯扎溴铵工作液直接接触rhColⅢ可导致其发生聚集。The disinfectants available for facial injectable fillers include 75%ethanol,iodinecontaining and surfactant-based disinfectants.There is a lack of research on whether the use of various skin disinfectants influences the activity of recombinant typeⅢhumanized collagen(rhColⅢ).The toxicity of 75%ethanol,iodophor disinfectant,chlorhexidine gluconate,benzalkonium chloride,and benzalkonium bromide on cells and the cell adhesion activity of rhColⅢwere investigated by CCK8 experiments to predict the effect of disinfectant on collagen activity during facial fillers,in order to provide guidance for clinical application.The results show that all disinfectants have cytotoxicity to NIH/3T3 mouse embryonic fibroblasts and HFB human skin fibroblasts,and the comparative results rank as chlorhexidine gluconate<benzalkonium chloride and benzalkonium bromide<75%ethanol and iodophor disinfectant.When the stock solution of each disinfectant is diluted 100 times,the effect on the cell adhesion activity of rhColⅢranks as follows:chlorhexidine gluconate and benzalkonium chloride<75%ethanol<iodophor and benzalkonium bromide.Both iodophor and benzethonium bromide working solutions significantly reduce the pro-cell adhesion activity of rhColⅢ.Iodophor,chlorhexidine gluconate,and benzethonium bromide working solutions cause aggregation of rhColⅢ.

关 键 词：重组人源化胶原蛋白 填充 消毒剂 细胞毒性 细胞黏附活性 

分 类 号：TQ658[化学工程—精细化工]