不同牡丹品种花粉的可溶性糖、可溶性蛋白含量和抗氧化酶活性分析  

Analysis of Soluble Sugar,Soluble Protein Content and Antioxidant Enzyme Activity in Pollen of Different Tree Peony Varieties

作　　者：李新伟 李婷 张镇梁 张彤 张峥 乔琦[1] 侯小改[1] LI Xinwei;LI Ting;ZHANG Zhenliang;ZHANG Tong;ZHANG Zheng;QIAO Qi;HOU Xiaogai(Agricultural College/Peony College,Henan University of Science and Technology,Luoyang 471023,China)

机构地区：[1]河南科技大学农学院/牡丹学院,河南洛阳471000

出　　处：《浙江林业科技》2023年第4期57-62,共6页Journal of Zhejiang Forestry Science and Technology

摘　　要：以早花、中花、晚花16个牡丹Paeonia suffruticosa栽培品种的花粉为材料,采用蒽酮比色法和凯氏定氮法分别测定其可溶性糖和可溶性蛋白含量,并比较不同温度下花粉的超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性变化。结果表明,不同花期的牡丹品种花粉中的可溶性糖和可溶性蛋白含量存在显著差异(P<0.05),以中花品种花粉中的可溶性蛋白含量较高,晚花品种花粉中的可溶性糖含量较高,其中,中花品种‘肉芙蓉’花粉中的可溶性蛋白含量最高,达1.35 mg·g^(-1),晚花品种‘五大洲’花粉中的可溶性糖含量最高,达74.19mg·g^(-1);不同温度贮藏24h后,各牡丹品种花粉的抗氧化酶活性不同,大部分品种花粉的SOD和POD活性随着温度的降低逐渐增加,而CAT活性的变化趋势则复杂多变。由于大部分品种的SOD和POD酶活性在4℃时较高,因此推荐4℃为牡丹花丝茶的常规保存温度。During April and May 2020,pollen of 16 cultivars of Paeonia×suffruticosa was collected from Luoyang,Henan province to determine soluble sugar and soluble protein content,as well as superoxide dismutase(SOD),peroxidase(POD)and catalase(CAT)activities of pollen stored under different temperatures.The results showed that there were significant differences among cultivars with different flowering stages,and the mid-flowering cultivars were dominant in soluble protein and the late-flowering ones in soluble sugar content.The soluble protein content of the mid-flowering cultivar P.×suffruticosa‘Roufurong’was the highest,1.35 mg/g and the late-flowering one P.×suffruticosa‘Wudazhou’was the highest,74.19 mg/g.After 24 hours of storage under different temperatures,the antioxidant enzyme activities of each cultivar were different.The activities of SOD and POD enzymes in most cultivars increased gradually with the decrease of temperature,while the change trend of CAT enzyme activity was more complex.SOD and POD activities of most cultivars were Relative higher at 4℃.

关 键 词：牡丹 花粉 可溶性糖 可溶性蛋白 抗氧化酶 

分 类 号：S685.11[农业科学—观赏园艺]