生育哺乳对二甲基苯蒽诱导雌性SD大鼠乳腺癌发生的影响  

Effect of fertility and lactation on dimethylbenthracene-induced breast cancer in female SD rats

作　　者：潘吴媛 冯叶彬[2] 吴鑫垚 王梅 李志雄 涂书清 PAN Wuyuan;FENG Yebin;WU Xinyao;WANG Mei;LI Zhixiong;TU Shuqing(Department of Gynaecology,Fujian Maternity and Children Health Hospital,Fujian Province,Fuzhou350001,China;Department of Science and Education,Fujian Maternity and Children Health Hospital,Fujian Province,Fuzhou350001,China;School of Basic Medical,Fujian Medical University,Fujian Province,Fuzhou350004,China;Laboratory Center for Non Human Primates,Fujian Maternity and Children Health Hospital,Fujian Province,Fuzhou350001,China)

机构地区：[1]福建省妇幼保健院妇科,福建福州350001 [2]福建省妇幼保健院科教科,福建福州350001 [3]福建医科大学基础医学院,福建福州350004 [4]福建省妇幼保健院非人灵长类动物实验中心,福建福州350001

出　　处：《中国当代医药》2023年第20期19-22,F0003,共5页China Modern Medicine

基　　金：福建省科技计划项目(2020R1012-1)。

摘　　要：目的研究生育哺乳是否能够降低二甲基苯蒽(DMBA)诱导雌性SD大鼠乳腺癌发生的概率。方法采用随机数表法将40只8周龄雌性SD处鼠随机分为未生育对照组和未生育DMBA组各20只,将80只36周龄雌性SD处鼠随机分为生育未哺乳对照组、生育未哺乳DMBA组、生育哺乳对照组、生育哺乳DMBA组各20只。未生育DMBA组、生育未哺乳DMBA组、生育哺乳DMBA组给予10 mg/100 gDMBA灌胃(两次给药间隔2周)。未生育对照组、生育未哺乳对照组、生育哺乳对照组灌胃相同剂量的生理盐水作为平行对照,各组均正常饮食喂养。24周后各组大鼠全部处死,解剖并观察乳腺,核仁组成区嗜银蛋白(AgNOR)染色并计数,检测增殖细胞核抗原(PCNA)、Ki-67、人类表皮生长因子受体2(C-erb B-2)、微小染色体维持蛋白(MCM)2在大鼠乳腺上皮细胞中的表达,各组之间进行同期比较。结果生育哺乳DMBA组的发病率、潜伏期低于生育未哺乳DMBA组、未生育DMBA组,差异有统计学意义(P<0.001)。生育未哺乳DMBA组的发病率、潜伏期低于未生育DMBA组,差异有统计学意义(P<0.001)。三组肿瘤直径比较,差异无统计学意义(P>0.05)。未生育DMBA组、生育哺乳DMBA组、生育未哺乳DMBA组的AgNOR计数、C-erbB-2、PCNA、Ki-67、MCM2阳性表达率低于未生育对照组、生育未哺乳对照组、生育哺乳对照组,差异有统计学意义(P<0.001)。生育哺乳DMBA组、生育未哺乳DMBA组的核仁组成区嗜银蛋白计数低于未生育DMBA组,差异有统计学意义(P<0.001)。生育哺乳DMBA组的C-erb B-2、PCNA、Ki-67、MCM2阳性表达率低于生育未哺乳DMBA组、未生育DMBA组,差异有统计学意义(P<0.001)。结论生育哺乳能够有效降低二甲基苯蒽诱导雌性SD大鼠乳腺癌发生的概率。Objective To investigate whether reproductive lactation can reduce the probability of dimethylbenz anthrancence(DMBA)induced breast cancer in female SD rats.Methods Forty 8-week-old female SD rats were divided into two groups by using random number table method,20 in the unfertilized control group and 20 in the unfertilized DMBA group.Eighty 36-week-old female SD rats were randomly divided into fertile and non-lactating control group,fertile and non-lactating DMBA group,fertile and lactating control group,and fertile and lactating DMBA group with 20 rats each.The sterile DMBA group,fertile non-lactating DMBA group and fertile lactating DMBA group were given 10 mg/100 g DMBA by gavage(the interval between the two doses was 2 weeks).Non-fertility control group,childbearing non-lactation control group and childbearing and lactation control group were given the same dose of normal saline by gavage as parallel control.After the administration of DMBA for 24 weeks,the experimental group and the corresponding control group were all sacrificed,and the mammary glands were dissected to observe,stained and counted with argyrophilic nucleolar organizer region(AgNOR),and the expressions of proliferating cell nuclear antigen(PCNA),Ki-67,human epidermal growth factor receptor(C-erb B-2),minichromosome maintenance proteins(MCM)2 in rat mammary epithelial cells were detected,and comparisons were made between the groups over the same period.Results The incidence and incubation period of DMBA group were lower than the DMBA group and DMBA group,and the differences were statistically significant(P<0.05).The incidence and incubation period of DMBA group were lower than the DMBA group,and the differences were statistically significant(P<0.05).There was no significant difference in tumor diameter among the three groups(P>0.05).The AgNOR count of lactating DMBA group and non lactating DMBA group was lower than the non lactating DMBA group,the difference was statistically significant(P<0.05).The positive expression rates of C-erb-2,PCNA,K

关 键 词：生育哺乳 乳腺癌 二甲基苯蒽 动物模型 

分 类 号：R737.9[医药卫生—肿瘤]