烟熏法建立宫内发育迟缓模拟矮小症动物模型的研究进展  

Research progress on the establishment of intrauterine growth retardation simulating short stature animal model by cigarette smoking

作　　者：余风 明阳灿 徐辉甫 YU Feng;MING Yangcan;XU Huifu(The First Clinical College,Hubei University of Chinese Medicine,Hubei Province,Wuhan430061,China;Department of Pediatrics,Wuhan Hospital of Traditional Chinese and Western Medicine,Hubei Province,Wuhan430022,China)

机构地区：[1]湖北中医药大学第一临床学院,湖北武汉430061 [2]武汉市中西医结合医院儿科,湖北武汉430022

出　　处：《中国当代医药》2023年第20期33-36,45,共5页China Modern Medicine

基　　金：中华国际医学交流基金会中西医结合儿科中青年医师成长科研基金项目(Z-2019-41-2101-02)。

摘　　要：矮小症相关实验研究还不充分,尚未构建出标准化的动物模型。目前以宫内发育迟缓模型代替。烟熏法作为造宫内发育迟缓模型的常见方法,不同造模目的所需的烟熏条件不一。烟熏致宫内发育迟缓模拟矮小症的烟熏浓度、烟熏箱容积、孕鼠烟熏时长、烟熏周期等实验条件的缺乏统一标准。为了建立一个简便、经济、可重复性高的矮小症动物烟熏模型,检索中国生物医学文献服务系统(CBM)、中国知网、维普、万方数据、PubMed数据库烟熏制备宫内发育迟缓的文献,整理其中数据,对烟熏致大鼠宫内发育迟缓模型建立方法进行总结,收集相关数据并进行简要归纳分析。认为烟熏法制备宫内发育迟缓成功率较高,部分胎鼠符合矮小症诊断,目前可供参考的染毒箱大小为0.3~0.6 m^(3),需监测CO、CO_(2)、总悬浮颗粒(TSPs)浓度。本文就烟熏法制备宫内发育迟缓矮小症动物模型研究进展做一综述。The experimental research on short stature is insufficient,and the standardized animal model has not been structured yet.At present,intrauterine growth retardation model is used instead.As a common method to establish intrauterine growth retardation model,smoking conditions are different.The experimental conditions of intrauterine growth retardation caused by smoking,such as the concentration of smoke,the volume of smoke box,the duration of smoking in pregnant rats,and the smoking cycle,lack of unified standards.In order to establish a simple,economical and repeatable short stature animal model,we searched Chinese BioMedical Literature Database(CBM),CNKI,Vip,Wanfang data and PubMed database to prepare the literature of intrauterine growth retardation by smoking,sorted out the data,summarized the establishment method of intrauterine growth retardation model in rats caused by smoking,collected data and briefly summarized and analyzed them.It is considered that the success rate of building intrauterine growth retardation by smoking is high,and some fetal rats are consistent with the diagnosis of short stature.At present,the proper size of the exposure box is 0.3-0.6 m^(3),and the concentration of CO,CO_(2) and total suspended particulates(TSPs)needs to be monitored.This article reviews the research progress in establishing intrauterine growth retardation animal models by smoking to simulate animal model of short stature.

关 键 词：矮小症 动物模型 烟熏法 宫内发育迟缓 

分 类 号：R332[医药卫生—人体生理学]