Laboratory diagnosis of nonpolio enteroviruses:A review of the current literature  

作　　者：Tarek Itani Vladislav Chalapa Aleksandr Semenov Aleksandr Sergeev 

机构地区：[1]Yekaterinburg Research Institute of Viral Infections,SRC VB VEKTOR,Rospotrebnadzor,Yekaterinburg,Russia [2]Ural State Medical University,Yekaterinburg,Russia

出　　处：《Biosafety and Health》2023年第2期112-119,共8页生物安全与健康（英文）

基　　金：This study was funded by the Russian Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing,grant ID 121041500041−1.

摘　　要：Infections by nonpolio enteroviruses(EVs)are highly prevalent,particularly among children and neonates,where they may cause substantial morbidity and mortality.Laboratory diagnosis of these viral infections is important in patient prognosis and guidance of clinical management.Although the laboratory diagnosis of non-polio EVs is mainly based on molecular techniques,classical virus-isolation techniques are still used in refer-ence laboratories.Other techniques,such as antigen detection and serology,are becoming obsolete and rarely used in diagnosis.An important part of diagnosis and surveillance of EV infections is viral typing by VP1 gene sequencing using conventional Sanger technique and more recently,full-genome next-generation sequencing.The latter allows the typing of all EVs,better investigation of EV outbreaks,detection of coinfec-tion,and identification of severity markers in the EV genome.

关 键 词：Nonpolio enteroviruses Virus isolation Nucleic acid amplification tests Sanger sequencing Next-generation sequencing 

分 类 号：R446.5[医药卫生—诊断学]