利用汞膜修饰电极对培养基环境下成肌细胞吸收Cu2+的定量分析研究  

Quantitative Analysis of Cu2+Absorption by Myoblasts in Culture Medium Via the Mercury-film Modified Electrode

作　　者：朱晓璐[1] 程浩 赵文杰 尹曦 卢胜波 Zhu Xiaolu;Cheng Hao;Zhao Wenjie;Yin Xi;Lu Shengbo(College of Mechanical&Electrical Engineering,Hohai University,Changzhou,China;Kondas(Nanjing)Technology Co.,Ltd.,Nanjing,China)

机构地区：[1]河海大学机电工程学院,江苏常州 [2]康达斯(南京)科技有限公司,江苏南京

出　　处：《科学技术创新》2023年第18期21-24,共4页Scientific and Technological Innovation

基　　金：国家自然科学基金项目(51875170);常州市科技支撑计划(社会发展)项目(CE20195037)。

摘　　要：本文提出针对细胞培养基的特性,通过优化的差分脉冲溶出伏安法测量成肌细胞对铜离子的吸收量。在三电极体系中采用120 mg/L的Hg2+在-0.2 V下对工作电极镀膜,实现检测铜离子最低0.5 ng/mL的浓度变化量,本方法对细胞培养基内铜离子的线性测量范围约为1-150 ng/mL,且溶出峰约在-0.20 V至-0.15 V之间,并具有高度选择性和快速特性。试验结果表明,在初始外加10和50 ng/mL Cu^(2+)的含血清培养基中,成肌细胞3天后对其吸收度分别为15.2和29.6 ng/ml。本文亦讨论了在培养基条件测得的Cu^(2+)溶出峰相对水溶液发生左移的可能机制。This study proposes the method for measuring absorption amount of Cu^(2+)by myoblast cells during growing by the optimized differential pulse stripping voltammetry.In this three-electrode system,120 mg/L Hg2+was employed to coating the working electrode at-0.2 V to quantitatively detect Cu^(2+)at the minimum variation of 0.5 ng/mL in cell culture medium within 1-150 ng/mL.The stripping peak of copper in the medium was between-0.20 V and-0.15 V,and it was highly selective and performed rapidly.The results show the absorbability of Cu^(2+)is 15.2 ng/mL and 29.6 ng/mL in serum-contained medium after 3 days,initially added with 10 and 50 ng/mL Cu^(2+),respectively.The possible mechanism of the left shift of the stripping peak of Cu^(2+)measured in the medium relative to aqueous solution was also discussed.

关 键 词：电化学三电极体系 阳极溶出伏安法 细胞培养基 铜 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]