UPLC-MS/MS法同时测定乌灵胶囊/乌灵菌粉中10种核苷  

Simultaneous determination of ten nucleosides in Wuling Capsules/Wuling fungus powder by UPLC-MS/MS

作　　者：黄小凤 饶亮明 青琳森[1] 梁健[1] 姚瑶 HUANG Xiao-feng;RAO Liang-ming;QING Lin-sen;LIANG Jian;YAO Yao(Chengdu Institute of Biology,Chinese Academy of Sciences,Chengdu 610041,China;Shanghai Institute of Nutrition and Health,Chinese Academy of Sciences,Shanghai 200031,China;Zhejiang Jolly Pharmaceutical Co.,Ltd.,Huzhou 313200,China)

机构地区：[1]中国科学院成都生物研究所,四川成都610041 [2]中国科学院上海营养与健康研究所,上海200031 [3]浙江佐力药业股份有限公司,浙江湖州313200

出　　处：《中成药》2023年第7期2144-2148,共5页Chinese Traditional Patent Medicine

基　　金：四川省科技计划项目(2022YFS0011);中国科学院大学生科创计划资助项目(20214002464)。

摘　　要：目的建立UPLC-MS/MS法同时测定乌灵胶囊/乌灵菌粉中腺苷、胞苷、鸟苷、肌苷、尿苷、脱氧鸟苷、胞嘧啶核苷酸、尿嘧啶核苷酸、鸟嘌呤核苷酸、腺嘌呤核苷酸的含量。方法该药物水提液的分析采用Thermo Hypercard色谱柱(2.1 mm×100 mm,5μm);流动相水(含2 mmol/L乙酸铵、0.06%乙二胺)-乙腈(含2 mmol/L乙酸铵、0.06%乙二胺),梯度洗脱;体积流量0.6 mL/min;柱温40℃;电喷雾离子源;负离子扫描;多反应监测模式。结果10种成分在各自范围内线性关系良好(R^(2)>0.9900),平均加样回收率85.52%~104.21%,RSD 0.79%~4.48%。结论该方法简便可行,重复性好,可用于乌灵胶囊/乌灵菌粉的质量控制。AIM To establish a UPLC-MS/MS method for the simultaneous content determination of adenosine,cytidine,guanosine,inosine,uridine,deoxyguanosine,cytidine monophosphate,uridine monophosphate,guanosine monophosphate and adenosine monophosphate in Wuling Capsules/Wuling fungus powder.METHODS The analysis of aqueous extract of this drug was performed on a 40℃thermostatic Thermo Hypercard column(2.1 mm×100 mm,5μm),with the mobile phase comprising of water(containing 2 mmol/L ammonium acetate,0.06%ethylenediamine)-acetonitrile(containing 2 mmol/L ammonium acetate,0.06%ethylenediamine)flowing at 0.6 mL/min in a gradient elution manner,and electron spray ionization source was adopted in negative ion scanning with multiple reaction monitoring mode.RESULTS Ten constituents showed good linear relationships within their own ranges(R^(2)>0.9900),whose average recoveries were 85.52%-104.21%with the RSDs of 0.79%-4.48%.CONCLUSION This simple,feasible and reproducible method can be used for the quality control of Wuling Capsules/Wuling fungus powder.

关 键 词：乌灵胶囊 乌灵菌粉 核苷 UPLC-MS/MS 

分 类 号：R927.2[医药卫生—药学]