田基黄化学成分及其抗骨质疏松活性  

Chemical constituents from Hypericum japonicum and their anti-osteoporotic activities

作　　者：程丽娟[1] 王凯 CHENG Li-juan;WANG Kai(Yichun Vocational Technical College,Yichun 336000,China;College of Pharmacy,Jiangxi University of Chinese Medicine,Nanchang 330004,China)

机构地区：[1]宜春职业技术学院,江西宜春336000 [2]江西中医药大学药学院,江西南昌330004

出　　处：《中成药》2023年第7期2234-2238,共5页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金(82160737)。

摘　　要：目的研究田基黄Hypericum japonicum Thunb.ex Murray化学成分及其抗骨质疏松活性。方法田基黄提取物采用硅胶、Sephadex LH-20、半制备HPLC进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构。采用RANKL诱导小鼠骨髓单核巨噬细胞BMM形成的成熟破骨细胞模型,筛选各化合物抗骨质疏松活性。结果从中分离得到7个化合物,分别鉴定为田基黄素A(1)、(R,Z)-2-(4,8-dimethylnona-3,7-dien-1-yl)-2,7-dimethyl-2 H-chromen-5-ol(2)、benzyl 1-O-β-D-glucopyranoside(3)、染料木黄酮(4)、杨梅素-3-O-β-D-半乳糖苷(5)、(E)-4-[5-(hydroxymethyl)furan-2-yl]but-3-en-2-one(6)、5-羟甲基糠醛(7)。化合物1、4~5的破骨细胞存活率分别为(15.34±0.05)%、(26.75±0.18)%、(21.93±0.14)%。结论化合物1为新化合物,化合物2为新天然产物,化合物3~7为首次从该植物中分离得到。化合物1、4~5具有良好的破骨细胞抑制活性。AIM To study the chemical constituents from Hypericum japonicum Thunb.ex Murray and their anti-osteoporotic activities.METHODS The extract from H.japonicum was isolated and purified by silica gel,Sephadex LH-20l and semi-preparative HPLC,then the structures of obtained compounds were identified by physicochemical properties and spectral data.The anti-osteoporosis activities of compounds were screened by using RANKL induced mouse bone marrow mononuclear macrophage cell BMM to mature osteoclast model.RESULTS Seven compounds were isolated and identified as hyperjapsin A(1),(R,Z)-2-(4,8-dimethylnona-3,7-dien-1-yl)-2,7-dimethyl-2 H-chromen-5-ol(2),benzyl 1-O-β-D-glucopyranoside(3),genistein(4),myricetin-3-O-β-D-galactopyranoside(5),(E)-4-[5-(hydroxymethyl)furan-2-yl]but-3-en-2-one(6),5-hydroxymethyl-2-furancarboxaldehyde(7).The survival rates of osteoclast of compounds 1,4-5 were(15.34±0.05)%,(26.75±0.18)%,(21.93±0.14)%,respectively.CONCLUSION Compound 1 is a new compound.Compound 2 is a new natural product.Compounds 3-7 are isolated from this plant for the first time.Compounds 1,4-5 display significant anti-osteoclasts activities.

关 键 词：田基黄 化学成分 分离鉴定 抗骨质疏松活性 

分 类 号：R284.1[医药卫生—中药学]