多级好氧生物膜/厌氧颗粒反应器的PN/A工艺启动与调控  

Start-up and regulation of PN/A process in a multi-stage aerobic-biofilm/anoxic-particle reactor

作　　者：赵杰俊 ZHAO Jiejun(CCCC(Tianjin)Eco-Environmental Protection Design&Research Institute Co,Tianjin 300000,China)

机构地区：[1]中交(天津)生态环保设计研究院有限公司,天津300000

出　　处：《环境工程学报》2023年第5期1683-1694,共12页Chinese Journal of Environmental Engineering

摘　　要：厌氧氨氧化细菌(AnAOB)和氨氧化细菌(AOB)的同时快速富集是短程硝化/厌氧氨氧化(PN/A)工艺高效运行的关键。本研究构建了一种多级好氧生物膜/厌氧颗粒污泥反应器(MOBAPR)。MOBAPR的好氧生物膜/厌氧颗粒结构不仅可有效保留生物质,还为AnAOB和AOB同时富集创造空间条件。在MOBAPR中,首先通过控制pH和间歇曝气在15 d内实现PN工艺的快速启动,然后在稳定运行的PN工艺基础上,61 d后实现了PN/A工艺的快速启动,脱氮率达到(83.41±2.45)%。16S rRNA基因测序结果表明,MOBAPR中AOB(27.09%)和AnAOB(9.99%)成功实现同时富集。此外,在PN/A工艺运行过程中,气液比(曝气速率与进水速率的比值)是一个与NRE高度相关的操作参数。气液比的优化控制不仅可以提高MOBAPR的脱氮性能,还可以在溶解氧(DO)低至无法控制时代替DO控制。The simultaneous and rapid enrichment of anaerobic ammonia-oxidizing bacteria(AnAOB)and ammonia-oxidizing bacteria(AOB)is the key to efficiently perform the partial nitrification/anaerobic ammonia oxidation(PN/A)process.In this study,a multistage aerobic-biofilm/anaerobic-granular sludge reactor(MOBAPR)was constructed.The aerobic-biofilm/anaerobic-particle structure of MOBAPR not only effectively retains biomass,but also provides the spatial conditions for simultaneous enrichment of AnAOB and AOB.In MOBAPR,fast start-up of the PN process was achieved by controlling pH and intermittent aeration within 15 days.Then on the basis of the stable running of PN process,the rapid start of PN/A process was accomplished 61 days later,and the nitrogen removal efficiency(NRE)reached(83.41±2.45)%.16S rRNA gene sequencing revealed that the simultaneous enrichment of AOB(27.09%)and AnAOB(9.99%)in MOBAPR occurred.During the operation of the PN/A process,The Gas/Liquid ratio(aeration rate to water inflow rate)was an operational parameter highly correlated(0.992)with NRE.The optimal control of G/L can not only improve the nitrogen removal performance of MOBAPR,but also replace DO control when DO is too low to control.

关 键 词：短程硝化 厌氧氨氧化 自养脱氮 微生物特性 

分 类 号：X703[环境科学与工程—环境工程]