聚瓜氨酸修饰电极测定烟酰胺  

Determination of nicotinamide at polycitrulline modified electrodes

作　　者：尹明静 张秀玉 王宜磊[2] 马心英 陈美凤 YIN Ming-jing;ZHANG Xiu-yu;WANG Yi-lei;MA Xin-ying;CHEN Mei-feng(College of Chemistry and Chemical Engineering,Heze University,Heze 274015,China;College of Agriculture and Bioengineering,Heze University,Heze 274015,China)

机构地区：[1]菏泽学院化学化工学院,山东菏泽274015 [2]菏泽学院农业与生物工程学院,山东菏泽274015

出　　处：《现代化工》2023年第6期249-253,共5页Modern Chemical Industry

基　　金：山东省自然科学基金项目(ZR2014BL020);山东省高等学校协同创新计划项目。

摘　　要：通过电聚合的方法在玻碳电极表面形成聚瓜氨酸膜(PCit/GCE),制备了聚瓜氨酸玻碳电极,用于测量烟酰胺(NA),建立了测定烟酰胺的新方法。结果表明,利用PCit/GCE测定烟酰胺有较高的灵敏度,还原峰电流明显增强,烟酰胺检出浓度4.0×10^(-7)mol/L。烟酰胺浓度还原峰电流在4.00×10^(-6)~1.00×10^(-4)mol/L范围内有良好的线性关系,线性方程式为i_(pc)(A)=5.32×10^(-7)+0.36c(mol/L),相关系数R为0.9939。PCit/GCE修饰电极测定护肤品和奶粉中的NA,回收率分别为96.1%、97%。Polycitrulline(PCIT)modified glass-carbon electrode(PCIT/GCE)is obtained through forming polycitrulline film on the surface of a glass-carbon electrode(GCE)by electro-polymerization method,and used to detect nicotinamide,which represents a new method for the determination of niacinamide.It is found that PCIT/GCE has a higher sensitivity in detecting nicotinamide with a significantly enhanced reduction peak current.The peak current appears at a nicotinamide concentration of 4.0×10^(-7)mol·L^(-1).It is verified there is a good liner relationship between current intensity and nicotinamide concentration in the range of 4.00×10^(-6)-1.00×10^(-4)mol·L^(-1).The linear equation is i_(pc)(A)=5.32×10^(-7)+0.36c(mol·L^(-1)),and the correlation coefficient is 0.9939.The recoveries are 96.1%and 97%,respectively when PCIT/GCE modified electrode is used to determine nicotinamide in skin care products and milk powder.

关 键 词：瓜氨酸 玻碳电极 烟酰胺 循环伏安法 

分 类 号：O65[理学—分析化学]