厌氧序批式生物膜反应器处理葡萄酒生产废水性能及菌群演替分析  

Performance on treating winery wastewater by AnSBBR and analysis of the microbial community succession

作　　者：郑兰香 王泽 马芷萱 刘烨星 李功[1] 常洁 于莉芳[3] ZHENG Lanxiang;WANG Ze;MA Zhixuan;LIU Yexing;LI Gong;CHANG Jie;YU Lifang(College of Ecology and Environment,Ningxia University,Yinchuan 750021,China;China Wine Industry Technology Institute,Yinchuan 750021,China;School of Municipal and Environmental Engineering,Xi'an University of Architecture and Technology,Xi'an 710055,China;School of Geographical Science and Planning,Ningxia University,Yinchuan 750021,China)

机构地区：[1]宁夏大学生态环境学院,银川750021 [2]中国葡萄酒产业技术研究院,银川750021 [3]西安建筑科技大学环境与市政工程学院,西安710055 [4]宁夏大学地理科学与规划学院,银川750021

出　　处：《环境工程学报》2023年第4期1387-1396,共10页Chinese Journal of Environmental Engineering

基　　金：国家重点研发计划项目(2019YFD1002500);宁夏科技厅重点研发计划项目(2019BBF02024)。

摘　　要：为探究厌氧序批式生物膜法处理葡萄酒生产废水的可行性,采用厌氧序批式生物膜反应器(AnSBBR)预处理模拟葡萄酒生产废水,通过逐级增加进水浓度来提升有机负荷并对AnSBBR在不同负荷下的运行特性及菌群演替规律。结果表明:在35℃、HRT为20 h、周期为4 h的运行工况下,当OLR为1.2~9.6 g·(L·d)-1时,AnSBBR均能保证出水COD值低于200 mg·L^(-1),当OLR为5.4 g·(L·d)^(-1)时,运行性能最佳,COD去除率为(97.2±0.5)%,甲烷产率为(349.9±7.6) mL·g^(-1);与OLR为1.2 g·(L·d)^(-1)相比,OLR为9.6 g·(L·d)^(-1)时产甲烷速率提高69.3%,生物量增加294.3%,辅酶F420浓度增加190.8%,电子传递活性(INT-ETS)提高88.4%;当OLR增至10.2 g·(L·d)^(-1)后,反应器中VFA浓度持续增加,丙酸和丁酸积累,缓冲能力明显下降,系统无法适应该负荷条件;高通量测序显示,群落中主要的细菌为Desulfovibrio、Brevinema、Treponema、Longilinea、Paludibacter和Leptolinea,主要的古菌为Methanobacterium、Methanobrevibacter,Methanosaeta和Methanosarcina;受进水基质组分和负荷的影响,细菌和古菌中丰度占比最大的分别为Desulfovibrio(12.4%)和Methanobacterium(17.1%);当OLR为5.4 g·(L·d)^(-1)时,产甲烷菌多样性更高且在门和属水平上整体丰度最大,分别为26.7%和26.3%;当OLR为9.6 g·(L·d)^(-1)时,培养出特定的菌属(Methanobrevibacter)以适应环境。由此可以看出,逐级提升OLR的运行策略可促进生物膜系统的增殖和代谢活性。本研究确定了AnSBBR处理葡萄酒生产废水的最大及最佳运行负荷,该结果可为推动AnSBBR的工程化应用提供依据。In order to explore the feasibility of treating winery wastewater by anaerobic sequencing biofilm batch reactor(AnSBBR),a laboratory-scale AnSBBR was used to pretreat the simulated winery wastewater.The organic loading rate was achieved by the gradual increase of the influent concentration,and its operational characteristics and microbial community composition at different loading rates were studied.The results showed that at 35℃,HRT of 20 h and operation period of 4 h,the effluent COD of AnSBBR was lower than 200 mg·L^(−1)when the OLR was 1.2~9.6 g·(L·d)^(−1).When the OLR was 5.4 g·(L·d)^(−1),the COD removal rate was 97.2±0.5%,the methane yield was 349.9±7.6 mL·g^(−1).Compared with the OLR of 1.2 g·(L·d)^(−1),the methanogenic rate increased by 69.3%,the biofilm biomass increased by 294.3%,the content of coenzyme F420 increased by 190.8%,and the activity of INT-ETS increased by 88.4%at the OLR of 9.6 g·(L·d)^(−1).When the OLR reached 10.2 g·(L·d)^(−1),the volatile fatty acids(VFA)concentration in AnSBBR continued to increase,the propionic acid and butyric acid gradually accumulated,and the buffer capacity significantly decreased,indicating that the AnSBBR system could note adopt the OLR of 10.2 g·(L·d)^(−1).Illumina Miseq sequencing showed that the dominant bacteria were Desulfovibrio,Brevinema,Treponema,Longilinea,Paludibacter,and Leptolinea,the dominant archaea were Methanobacterium,Methanobrevibacter,Methanosaeta,and Methanosarcina.The relative abundances of Desulfovibrio(12.4%)and Methanobacterium(17.1%)were the largest because the microbial community structure was affected by the characteristics of the influent matrix.When the OLR was 5.4 g·(L·d)^(−1),the diversity of archaea was higher,and the abundances were the largest at the level of phylum and genus with 26.7%and 26.3%,respectively.At the OLR of 9.6 g·(L·d)^(−1),the relative abundance of methanobacterium and methanosarcina were reduced and specific archaea(Methanobrevibacter)was cultivated to adapt to the enviro

关 键 词：葡萄酒生产废水 厌氧序批式生物膜反应器 有机负荷 胞外聚合物 群落结构 

分 类 号：X703[环境科学与工程—环境工程]