基于网络药理学分析虎骨治疗骨质疏松症的分子机制  

Molecular Mechanism of Tiger Bone Treatment for Osteoporosis Based on Network Pharmacology Analysis

作　　者：梁爽[1] 潘颖[1] LIANG Shuang;PAN Ying(The Third Hospital of Hebei Medical University,Shijiazhuang 050051,Hebei,Chnia)

机构地区：[1]河北医科大学第三医院,河北石家庄050051

出　　处：《实用中医内科杂志》2023年第3期35-37,I0005-I0007,共6页Journal of Practical Traditional Chinese Internal Medicine

基　　金：河北省中医药管理局科研计划项目(2020183)。

摘　　要：目的预测虎骨治疗骨质疏松症(OP)的靶点,探讨其治疗骨质疏松的分子作用机制。方法查阅文献,检索虎骨的化学成分和作用靶点,利用GeneCards和NCBI以及其他数据库中检索骨质疏松症相关基因。利用GO数据库和KEGG数据库进行分析富集,采用Cytoscape 3.8.0绘制中药-成分-疾病-途径-靶点网络图,并用分子对接对关键靶点进行验证。结果成分-靶标关系网络包括19个成分和168个靶标,包括关键靶标PTGS2、EGFR等。GO功能富集分析包括1259个生物过程,147个与分子功能相关,80个与细胞组分相关。通过对KEGG信号通路的富集和筛选,获得了26条信号通路。分子对接结果表明,预测的活性物质成分和关键靶点均具有较好地结合活性。结论虎骨中的氨基酸类等活性成分主要通过PTGS2和EGFR等靶点调节相关通路治疗骨质疏松症。反映了虎骨“多成分、多靶点、多途径”的功能特点。Objective To predict the target of tiger bone for osteoporosis(OP)and explore the molecular mechanism of osteoporosis.Methods Review the literature,retrieve the chemical composition and action targets of tiger bone,and retrieve osteoporosis-related genes using GeneCards and NCBI and other databases.Analytical enrichment was performed using the GO database and the KEGG database,and the traditional Chinese medicine-component-disease-pathway-target network map was drawn using Cytoscape 3.8.0,and the key targets were validated with molecular docking.Results The component-target relationship network includes 19 components and 168 targets,including the key targets,PTGS2,EGFR,etc.The GO functional enrichment analysis included 1,259 biological processes,147 associated with molecular function,and 80 associated with cell fractions.Through the enrichment and screening of the KEGG signaling pathway,26 signaling pathways were obtained.Molecular docking results indicate that both the predicted active material components and key targets have good binding activity.Conclusion Active components such as amino acids in tiger bone mainly regulate PTGS2 and EGFR.This study reflects the functional characteristics of"multi-component,multi-target and multipathway"of tiger bone.

关 键 词：虎骨 骨质疏松 靶点 信号通路 网络药理学 

分 类 号：R285[医药卫生—中药学]