裂解性噬菌体内溶酶和穿孔素抗革兰阴性菌的研究进展  

Research Progress of Lytic Phage Endolysin and Holin Against Gram-negative Bacteria

作　　者：荣睿璠 曾君 郭志良 左君豪 赵瑞利 郭海悦 孙旭阳 RONG Ruifan;ZENG Jun;GUO Zhiliang;ZUO Junhao;ZHAO Ruili;GUO Haiyue;SUN Xuyang(College of Animal Science and Veterinary Medicine,Tianjin Agricultural University/Tianjin Key Laboratory of Agricultural Animal Breeding and Healthy Breeding,Tianjin 300392,China)

机构地区：[1]天津农学院动物科学与动物医学学院/天津市农业动物繁育与健康养殖重点实验室,天津300392

出　　处：《畜牧兽医杂志》2023年第4期37-40,43,共5页Journal of Animal Science and Veterinary Medicine

基　　金：天津市应用基础与前沿技术研究计划一般项目(18JCYBJC30100、14JCYBJC30000);天津市高校“中青年骨干创新人才”培养计划;天津市奶牛(肉羊)、生猪产业技术体系创新团队建设项目(ITTCRS2021000);天津市天津市研究生科研创新项目(2021YJSS140)。

摘　　要：随着细菌耐药情况的日益严重,人们开始寻找抗生素的代替物,噬菌体内溶酶(endolysin)和穿孔素(holin)受到了关注。其中内溶酶展现了良好的抑菌潜力,与抗生素和噬菌体制剂相比有着一定的优点。而内溶酶的作用特点决定了其在抑制革兰阴性菌时需要穿孔素的协同,因此有研究利用基因工程把内溶酶和穿孔素进行重组来解决这一问题。此外,在噬菌体-细菌相互作用的研究中,已有研究表明噬菌体基因参与调控细菌间的通讯系统,内溶酶和穿孔素正是其中重要的调控因素。本文通过对噬菌体与噬菌体制剂的研究进展、内溶酶和穿孔素抗革兰阴性菌的研究进展及其应用进行了综述,以期为未来有关噬菌体裂解酶的研究提供思路。With the increasing severity of bacterial drug resistance,people began to look for alternatives to antibiotics,and bacteriophage endolysin and holin attracted attention.Among them,endolysin show good antibacterial potential and have certain advantages compared with antibiotics and bacteriophages.The action characteristics of endolysin determine that it needs the cooperation of holin when it inhibits gram-negative bacteria.Therefore,it has been studied to use genetic engineering to recombine endolysin and perforin to solve this problem.In addition,in the study of bacteriophage bacteria interaction,previous studies have shown that bacteriophage genes are involved in the regulation of bacterial communication system,of which endolysin and holin are important regulatory factors.This article reviews the research progress of bacteriophages and bacteriophage preparations,the research progress and application of endolysin and holin against gram-negative bacteria,with a view to providing ideas for future research on bacteriophage lyase.

关 键 词：裂解性噬菌体 革兰阴性菌 内溶酶 穿孔素 

分 类 号：S852.65[农业科学—基础兽医学]