万峰湖丰水期和枯水期细菌群落组成和多样性比较  

Comparative of bacterial community composition and diversity in the Wanfeng Lake between wet season and dry season

作　　者：秦燕 范波 梅丽 QIN Yan;FAN Bo;MEI Li(School of Biology and Chemistry,Minzu Normal University of Xingyi,Xingyi 562400,China)

机构地区：[1]兴义民族师范学院生物与化学学院,贵州兴义562400

出　　处：《大连海洋大学学报》2023年第3期414-422,共9页Journal of Dalian Ocean University

基　　金：贵州省教育厅青年科技人才成长项目(黔教合KY字[2019]221号);兴义民族师范学院博士科研基金(18XYBS02);黔西南州2019年州级科技计划自筹基金(2019-2-50)。

摘　　要：为比较贵州省黔西南州万峰湖丰水期和枯水期细菌群落组成和多样性,于2020年11月(丰水期)和2021年5月(枯水期)采集了万峰湖3个生态位点(大坝、梅家湾和巴结)表层(0~5 m)水体的水样,采用16S rRNA高通量测序技术分析其群落组成。结果表明:3个取样点丰水期细菌的OTUs共1647个,枯水期细菌的OTUs共1530个,枯水期细菌丰度高于丰水期,而丰水期细菌群落多样性高于枯水期;在门水平上,丰水期浮游细菌群落主要由变形菌门(Proteobacteria)、放线菌门(Actinobacteria)和蓝藻门(Cyanobacteria)等18门202属细菌组成,枯水期浮游细菌群落则主要由放线菌门、变形菌门和拟杆菌门(Bacteroidetes)等20门236属细菌组成;在属水平上,丰水期和枯水期共有属为55属,147属为丰水期特有,其中放线菌门的Nanopelagicaceae属、蓝菌属(Cyanobium)和羊栖菜弧菌属(Limnohabitans)丰度最高,181属为枯水期特有,其中蓝藻门的Gplla属、土生单胞菌属(Terrimonas)和玫瑰单胞菌属(Roseomonas)丰度最高;PICRUSt功能预测显示,大部分代谢途径中菌群预测的基因拷贝数为枯水期明显高于丰水期。研究表明,万峰湖丰水期和枯水期细菌群落组成和多样性存在明显差异,丰水期和枯水期均有其特有的细菌群落结构,且枯水期菌群代谢比丰水期更活跃。In order to compare the composition and diversity of bacterial community in wet and dry seasons of the Wanfeng Lake in Qianxian prefecture,Guizhou Province,water samples from the surface(0-5 m)of three ecological loci in the Wanfeng Lake were collected in November 2020(wet season)and May 2021(dry season),bacterial community composition of each sample was studied by 16S rRNA high-throughput sequencing technology.The sequence diversity and abundance indices showed that there were 1647 OTUs in the wet season and 1530 OTUs in the dry season.The relative bacterial abundance in the dry season was higher than that in the wet season.The bacterial community diversity in the wet season was higher than that in the dry season.The species classification results showed that the planktonic bacterial community in the wet season was mainly composed of 18 phylum(Proteobacteria,Actinobacteria and Cyanobacteria,etc.),and 202 genera of bacteria.The planktonic bacterial community in the dry season is mainly composed of 20 phylum(Actinobacteria,Proteobacteria and Bacteroidetes,etc.),and 236 genera of bacteria.The analysis of the genera showed that a total of 55 genera were common in both wet and dry seasons,and 147 genera were specific to the wet season,among which Nanopelagicaceae,Cyanobium and Limnohabitans of the Actinomycetes phylum had the higher abundance.181 genus is specific to the dry season,with the higher abundance of Gplla,Terramonas and Roseonas in the Cyanobacteria phylum.PICRUSt function prediction showed that the gene copy number in the wet season was significantly higher than that in the dry season in most metabolic pathways.The results reveal that there are significant differences in the composition and diversity of bacterial community between wet and dry seasons of the Wanfeng Lake,the wet and dry seasons have their own unique bacterial community structures,and the metabolism of bacterial community in the dry season is more active than that in the wet season.

关 键 词：万峰湖 细菌组成 16S rRNA 高通量测序 丰水期 枯水期 

分 类 号：S917[农业科学—水产科学] Q179.1[生物学—水生生物学]