Box-Behnken响应面法优选柴银颗粒中挥发油提取工艺  

Optimization of Extraction Process of Volatile Oil from Chaiyin Granules by Box-Behnken Design-Response Surface Methodology

作　　者：黄孟秋 邱荣英 李因坤 李倩 杜昊忱 HUANG Mengqiu;QIU Rongying;LI Yinkun;LI Qian;DU Haochen(Lunan Hope Pharmaceutical Co.,Ltd.,Linyi,Shandong,China 276006;State Key Laboratory of Generic Manufacture Technology of Traditional Chinese Medicine,Linyi,Shandong,China 276006;Shandong New Time Pharmaceutical Co.,Ltd.,Linyi,Shandong,China 276006)

机构地区：[1]鲁南厚普制药有限公司,山东临沂276006 [2]中药制药共性技术国家重点实验室<鲁南制药集团>,山东临沂276006 [3]山东新时代药业有限公司,山东临沂276006

出　　处：《中国药业》2023年第14期63-66,共4页China Pharmaceuticals

基　　金：山东省重点研发计划(重大科技创新工程)项目[2020CXGC010505];山东省新旧动能转换重大产业攻关项目[鲁动能办〔2021〕23号];山东省临沂市兰山区科技创新发展计划专项[2026]。

摘　　要：目的优选柴银颗粒中挥发油的提取工艺。方法在单因素试验基础上,以浸泡时间、加水量、提取时间为考察因素,以挥发油提取量为评价指标,采用Box-Behnken响应面法优选柴银颗粒中挥发油的提取工艺,并验证。结果最佳提取工艺为浸泡1.0 h,加10倍量水,提取4.0 h。在此工艺条件下,0.4倍处方量柴银颗粒可提取挥发油(2.60±0.03)mL,RSD为1.17%(n=3),与预测值的均方误差为0.0013。结论优选的提取工艺稳定、可行,可为柴银颗粒的工业化生产提供参考。Objective To optimize the extraction process of volatile oil from Chaiyin Granules.Methods Based on the single factor test,the extraction process of volatile oil from Chaiyin Granules was optimized by the Box-Behnken design-response surface methodology with the soaking time,water addition amount and extraction time as the investigation factors,and with the extraction amount of volatile oil as the evaluation index.Then,the verification test was carried out.Results The optimal extraction process was as follows:soaking for 1.0 h,adding 10 times the amount of water,and extracting for 4.0 h.Under the above conditions,(2.60±0.03)mL of volatile oil could be extracted from 0.4 times of the prescription amount,with the RSD of 1.17%(n=3),and the mean squared error between the real value and the predicted value was 0.0013.Conclusion The optimal extraction process is stable and feasible,which can provide a reference for the production process of Chaiyin Granules.

关 键 词：柴银颗粒 挥发油 Box-Behnken响应面法 提取工艺 工艺优选 

分 类 号：R943[医药卫生—药剂学]