柴胡皂苷类成分的肠吸收特性研究  

Study on the intestinal absorption characteristics of saikosaponins

作　　者：王雅芝 王启益 冯文忠 陈双双 孙欣光 周丽娟 张艳 张建永 段灿灿 WANG Yazhi;WANG Qiyi;FENG Wenzhong;CHEN Shuangshuang;SUN Xinguang;ZHOU Lijuan;ZHANG Yan;ZHANG Jianyong;DUAN Cancan(College of Pharmacy,Zunyi Medical University,Guizhou Zunyi563000,China;Beijing Zhendong Guangming Pharmaceutical Research Institute Co.,Ltd.,Beijing 100085,China;Key Lab of Basic Pharmacology of Ministry of Education&Joint International Research Laboratory ofEthnomedicine of Ministry of Education,Zunyi Medical University,Guizhou Zunyi 563000,China)

机构地区：[1]遵义医科大学药学院,贵州遵义563000 [2]北京振东光明药物研究院有限公司,北京100085 [3]遵义医科大学基础药理教育部重点实验室暨特色民族药教育部国际合作联合实验室,贵州遵义563000

出　　处：《中国药房》2023年第14期1681-1685,共5页China Pharmacy

基　　金：国家自然科学基金资助项目(No.82060754);遵义市优秀青年科技创新人才培养项目(No.遵优青科〔2021〕3号)。

摘　　要：目的探讨柴胡皂苷类成分的肠吸收特性。方法基于大鼠外翻肠囊模型,以累积吸收量(Q)、吸收速率常数(Ka)为指标,以超高效液相色谱-串联质谱技术为手段,检测柴胡总皂苷提取液(8 g/mL,以生药量计)中柴胡皂苷A、B_(2)、C、D、F在十二指肠、空肠、回肠中的吸收情况。结果柴胡皂苷A、B_(2)、C、F在十二指肠、空肠、回肠中吸收回归方程的相关系数(r)均大于0.95,柴胡皂苷D在上述肠段中的r均小于0.95;与同成分在十二指肠中的吸收比较,柴胡皂苷A、C在空肠、回肠中循环120 min时的Q、Ka以及柴胡皂苷F在回肠中循环120 min时的Q、Ka均显著降低(P<0.05)。结论柴胡皂苷A等5种柴胡皂苷类成分在十二指肠、空肠、回肠中均有吸收,其中柴胡皂苷A、B_(2)、C、F均为线性吸收,符合零级吸收特征;柴胡皂苷D为非线性吸收。OBJECTIVE To explore the intestinal absorption characteristics of saikosaponins.METHODS Based on everted intestinal sac model,using accumulative absorption amount(Q)and absorption rate constant(Ka)as indexes,UHPLC-MS/MS technique as a method,the absorption of saikosaponin A,B_(2),C,D and F from total saponins of Bupleurum chinense(8 g/mL,by crude drug)in the duodenum,jejunum and ileum was detected.RESULTS The correlation coefficients(r)of the regression equations for the absorption of saikosaponins A,B_(2),C and F in the duodenum,jejunum and ileum were all higher than 0.95,while the r of saikosaponin D in the above intestinal segments was lower than 0.95;compared with the absorption of the same composition in the duodenum,the Q and Ka of saikosaponin A and C circulating in jejunum and ileum for 120 min,as well as the Q and Ka of saikosaponin F circulating in the ileum for 120 min were significantly decreased(P<0.05).CONCLUSIONS Saikosaponin A and the other 4 saikosaponins are all absorbed in the duodenum,jejunum and ileum;among them,saikosaponin A,B_(2),C and F are linearly absorbed,which conforms to the zero-order absorption characteristics,but saikosaponin D shows nonlinear absorption.

关 键 词：柴胡 皂苷类成分 外翻肠囊法 累积吸收量 吸收速率常数 超高效液相色谱-串联质谱技术 

分 类 号：R969.1[医药卫生—药理学]