鬼针草总黄酮对脂多糖诱导小鼠神经炎症的改善作用及机制  

Improvement effects and mechanism of total flavonoids of Bidens pilosa on lipopolysaccharide-induced neuroinflammation in mice

作　　者：唐丰曼 庞晓军[1,2] 谢颜 刘春芳 TANG Fengman;PANG Xiaojun;XIE Yan;LIU Chunfang(College of Pharmacy,Guangxi MedicalUniversity,Nanning 530021,China;Dept.of Pharmacy,the Second People’s Hospital of Qinzhou,GuangxiQinzhou 535000,China)

机构地区：[1]广西医科大学药学院,南宁530021 [2]钦州市第二人民医院药学部,广西钦州535000

出　　处：《中国药房》2023年第14期1719-1723,共5页China Pharmacy

基　　金：钦州市科学研究与技术开发计划项目(No.202014810)。

摘　　要：目的探讨鬼针草总黄酮(TFB)对脂多糖(LPS)诱导小鼠神经炎症的改善作用及机制。方法将50只C57BL/6小鼠随机分为正常对照组、LPS组和TFB低、中、高剂量组,每组10只。TFB低、中、高剂量组小鼠按60、120、240 mg/kg灌胃TFB溶液,正常对照组和LPS组小鼠灌胃等体积生理盐水,每日1次,连续给药21 d。从给药第15天起,除正常对照组外,其余组小鼠连续7 d腹腔注射LPS(400μg/kg)建立神经炎症模型。末次给药4 h后麻醉小鼠取脑组织。观察小鼠神经元形态变化,检测小鼠脑组织中一氧化氮(NO)和肿瘤坏死因子α(TNF-α)、白细胞介素1β(IL-1β)、IL-6、IL-10的含量,检测小鼠脑组织中炎症通路相关蛋白[诱导型NO合酶(iNOS)、环氧合酶2(COX-2)、髓样分化因子88(Myd88)及蛋白激酶C(PKC)]的表达。结果与正常对照组相比,LPS组小鼠脑组织的海马区神经元排列稀疏紊乱,大量神经元固缩,核缩小;脑组织中TNF-α、IL-1β、IL-6、NO含量均显著增加,IL-10含量显著降低,iNOS、COX-2、Myd88、PKC蛋白相对表达量均显著升高(P<0.05)。与LPS组相比,TFB低、中、高剂量组小鼠脑组织神经元病理变化明显改善,脑组织中上述指标变化均显著逆转(P<0.05)。结论TFB具有抑制神经炎症的作用,其作用机制可能与下调炎症通路相关蛋白iNOS、COX-2、Myd88和PKC表达,减少炎症因子释放有关。OBJECTIVE To investigate the effect and mechanism of total flavonoids of Bidens pilosa L.(TFB)on lipopolysaccharide(LPS)-induced neuroinflammation in mice.METHODS Fifty C57BL/6 mice were randomly divided into normal control group,LPS group and TFB low-dose,medium-dose and high-dose groups,with 10 mice in each group.TFB low-dose,medium-dose and high-dose groups were given TFB solution intragastrically at 60,120 and 240 mg/kg,and the normal control group and LPS group were given corresponding volume of normal saline,once a day,for consecutive 21 d.From the 15th day of administration,except for the normal control group,other groups were given LPS(400μg/kg)intraperitoneally for 7 consecutive days to establish neuroinflammatory model.Brain tissues were taken under anesthesia 4 h after the final administration.The morphological changes of neuronal cells in mice were observed;the contents of nitric oxide(NO),tumor necrosis factorα(TNF-α),interleukin-1β(IL-1β),IL-6 and IL-10 were measured,and the expressions of inflammatory pathway-related proteins[inducible NO synthase(iNOS),cyclooxygenase-2(COX-2),myeloid differentiation factor 88(Myd88)and protein kinase C(PKC)]were measured in the brain tissues of mice.RESULTS Compared with the normal control group,the neuronal arrangement in the hippocampal region of the brain tissue of mice in the LPS group was sparsely disorganized,with a large number of neuronal fixations and shrunken nuclei;the contents of TNF-α,IL-1β,IL-6 and NO in the brain tissue were significantly increased,the contents of IL-10 were significantly decreased,and the relative expressions of iNOS,COX-2,Myd88 and PKC proteins were significantly increased(P<0.05).Compared with the LPS group,the neuronal pathological changes in the brain tissue of mice in the TFB low-dose,medium-dose and high-dose groups were significantly improved,and the changes of the above indices in the brain tissue were significantly reversed(P<0.05).CONCLUSIONS TFB has an inhibitory effect on neuroinflammation,and its mechanism of ac

关 键 词：鬼针草总黄酮 脂多糖 神经炎症 炎症因子 

分 类 号：R965[医药卫生—药理学]