HPLC法测定舒乐康洗液中4种药效成分含量  

Simultaneous Determination of Four Kinds of Active Ingredients in Shulekang Lotion By HPLC

作　　者：邢亚东 武敏 胡轲 孙大伟 李姗姗 XING Yadong;WU Min;HU Ke;SUN Dawei;LI Shanshan(Bengbu Medical College,Bengbu 233000,China;Suzhou First People's Hospital,Suzhou 234000,China;Taihe Institute for Food and Drug Control,Taihe 236600,China;Anhui Golden Sun Biochemical Pharmaceutical Co.,LTD,Yingshang 236000,China)

机构地区：[1]蚌埠医学院,安徽蚌埠233000 [2]宿州市第一人民医院,安徽宿州234000 [3]太和县食品药品检验所,安徽太和236600 [4]安徽金太阳生化药业有限公司,安徽颍上236000

出　　处：《西部中医药》2023年第7期39-42,共4页Western Journal of Traditional Chinese Medicine

基　　金：安徽省自然科学基金(1908085QH373);蚌埠医学院自然科学基金面上项目(BYKY1873)。

摘　　要：目的:建立舒乐康洗液中苍术素、盐酸小檗碱、蛇床子素、咖啡酸含量测定的高效液相色谱方法。方法:Syncronis C_(18)(250 mm×4.5 mm,4.6μm),检测波长:323 nm;乙腈(A)-0.02 moL/L磷酸二氢钾溶液(含0.2%三乙胺,磷酸溶液调pH值至2.83)(B);梯度洗脱;柱温:40℃;流速:1.0 mL/min。结果:苍术素、咖啡酸、盐酸小檗碱、蛇床子素分别在3.2317~48.4750μg(r=0.9998)、0.6474~9.7110μg(r=0.9996)、18.4200~276.3000μg(r=0.9998)和6.1467~92.2000μg(r=0.9997)范围内线性关系良好,平均加样回收率分别为99.37%、100.78%、99.48%和98.98%,RSD分别为1.09%、1.31%、0.97%和0.89%。结论:该法操作简便、稳定、重现性良好,结果准确,适合用于舒乐康洗液中苍术素、盐酸小檗碱、蛇床子素、咖啡酸含量分析。Objective:To establish a method of determining the contents of atrctylodin,berberine hydrochloride,osthole and caffeic acid contained in Shulekang lotion by HPLC.Methods:Syncronis C_(18)(250 mm×4.5 mm,4.6μm),detection wavelength:323nm;acetonitrile-0.02mol/L potassium dihydrogen phosphate solution(containing 0.2%triethylamine,adjust the pH value of the phosphate acid solution to 2.83);gradient elution;column temperature:40℃;current velocity:1.0 mL/min.Results:Atrctylodin,berberine hydrochloride,osthole and caffeic acid presented good linear relation in the range between 3.2317 and 48.4750μg(r=0.9998),0.6474 and 9.7110μg(r=0.9996),18.4200 and 276.3000μg(r=0.9998),6.1467 and 92.2000μg(r=0.9997)respectively,average sample recovery rates were 99.37%,100.78%,99.48%and 98.98%respectively,with RSD of 1.09%,1.31%,0.97%and 0.89%.Conclusion:The method,simple and convenient,stable,reproducible and accurate,is suitable for the content analysis of atrctylodin,berberine hydrochloride,osthole and caffeic acid contained in Shulekang lotion.

关 键 词：高效液相色谱法 舒乐康洗液 咖啡酸 苍术素 盐酸小檗碱 蛇床子素 含量测定 

分 类 号：R932[医药卫生—生药学]