Single-cell profiling reveals a potent role of quercetin in promoting hair regeneration  

作　　者：Qian Zhao Yandong Zheng Dongxin Zhao Liyun Zhao Lingling Geng Shuai Ma Yusheng Cai Chengyu Liu Yupeng Yan Juan Carlos Izpisua Belmonte Si Wang Weiqi Zhang Guang-Hui Liu Jing Qu 

机构地区：[1]Advanced Innovation Center for Human Brain Protection and National Clinical Research Center for Geriatric Disorders,Xuanwu Hospital Capital Medical University,Beijing 100053,China [2]Aging Translational Medicine Center,International Center for Aging and Cancer,Beijing Municipal Geriatric Medical Research Center,Xuan Wu Hospital,Capital Medical University,Beijing 100053,China [3]State Key Laboratory of Stem Cell and Reproductive Biology,Institute of Zoology,Chinese Academy of Sciences,Beijing 100101,China [4]University of Chinese Academy of Sciences,Beijing 100049,China [5]State Key Laboratory of Membrane Biology,Institute of Zoology,Chinese Academy of Sciences,Beijing 100101,China [6]Institute for Stem Cell and Regeneration,Chinese Academy of Sciences,Beijing 100101,China [7]Beijing Institute for Stem Cell and Regenerative Medicine,Beijing 100101,China [8]CAS Key Laboratory of Genomic and Precision Medicine,Beijing Institute of Genomics,Chinese Academy of Sciences,and China National Center for Bioinformation,Beijing 100101,China [9]Sino-Danish College,University of Chinese Academy of Sciences,Beijing 101408,China [10]The Fifth People's Hospital of Chongqing,Chongqing 400062,China [11]Shanghai Institute of Materia Medica,Chinese Academy of Sciences,Shanghai 201203,China [12]Altos Labs,Inc.,San Diego,CA 94022,USA

出　　处：《Protein & Cell》2023年第6期398-415,共18页蛋白质与细胞（英文版）

基　　金：supported by the National Key Research and Development Program of China(No.2020YFA0804000);the Strategic Priority Research Program of the Chinese Academy of Sciences(No.XDA16000000);the National Natural Science Foundation of China(Nos.82001477,81921006,82125011,92149301,92168201,91949209,92049304,92049116,32121001,32171447,82192863,82122024,82071588,32000500,81861168034,82271600 and 82201727);the National Key Research and Development Program of China(Nos.2018YFC2000100,2018YFA0107203,2020YFA0112200,2021YFF1201005,2021ZD0202401,2022YFA1103700 and 2021YFA1101401);CAS Project for Young Scientists in Basic Research(Nos.YSBR-076 and YSBR-012);the Program of the Beijing Natural Science Foundation(No.Z190019);K.C.Wong Education Foundation(Nos.GJTD-2019-06 and GJTD-2019-08);the Tencent Foundation(No.2021-1045);The Pilot Project for Public Welfare Development and Reform of Beijing-affiliated Medical Research Institutes(No.11000022T000000461062);Youth Innovation Promotion Association of CAS(Nos.E1CAZW0401 and 2022083);Young Elite Scientists Sponsorship Program by CAST(Nos.YESS20200012 and YESS20210002);the Informatization Plan of Chinese Academy of Sciences(Nos.CAS-WX2021SF-0301,CASWX2022SDC-XK14,and CAS-WX2021SF-0101);Beijing Hospitals Authority Youth Programme(No.QML20200802);the Open Research Program of State Key Laboratory of Membrane Biology(No.2021KF02);Grant from Key Laboratory of Stem Cells and Tissue Engineering(Sun Yat-Sen University),Ministry of Education(No.2021-A-001).

摘　　要：Hair loss affects millions of people at some time in their life,and safe and efficient treatments for hair loss are a significant unmet medical need.We report that topical delivery of quercetin(Que)stimulates resting hair follicles to grow with rapid follicular keratinocyte proliferation and replenishes perifollicular microvasculature in mice.We construct dynamic single-cell transcriptome landscape over the course of hair regrowth and find that Que treatment stimulates the differentiation trajectory in the hair follicles and induces an angiogenic signature in dermal endothelial cells by activating HIF-1αin endothelial cells.Skin administration of a HIF-1αagonist partially recapitulates the pro-angiogenesis and hair-growing effects of Que.Together,these findings provide a molecular understanding for the efficacy of Que in hair regrowth,which underscores the translational potential of targeting the hair follicle niche as a strategy for regenerative medicine,and suggest a route of pharmacological intervention that may promote hair regrowth.

关 键 词：single-cell RNA-sequencing QUE hair follicle regeneration endothelial cells HIF-1α 

分 类 号：Q2[生物学—细胞生物学]