基于多元统计分析的草珊瑚不同药用部位中10种成分含量的研究  

Content Determination of 10 Components in Different Medicinal parts of Sarcandra glabra Based on Multivariate Statistical Analysis

作　　者：王茜 廖华军[1] 鞠伟闽 贾雯 谢榕 郑莉丹 黄婧苡 潘馨[1] 王生华 WANG Qian;LIAO Hua-jun;JU Wei-min;JIA Wen;XIE Rong;ZHENG Li-dan;HUANG Jing-yi;PAN Xin;WANG Sheng-hua(School of Pharmacy,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China;Sanming Ludu Biotechnology Co.,Ltd.,Sanming 365000,China)

机构地区：[1]福建中医药大学药学院,福州350122 [2]三明绿都生物科技有限公司,福建三明365000

出　　处：《中国药学杂志》2023年第11期977-986,共10页Chinese Pharmaceutical Journal

基　　金：国家中医药管理局中医药行业科研专项资助(201507002-01-03)。

摘　　要：目的建立同时测定草珊瑚不同药用部位(全株、茎、叶)中富马酸、新绿原酸、绿原酸、隐绿原酸、咖啡酸、异嗪皮啶、槲皮素-3-O-β-D-葡萄糖醛酸苷、落新妇苷、山柰酚-3-O-β-D-葡萄糖醛酸苷、迷迭香酸等10种成分含量的方法,并比较差异。方法采用ChromCore AQ C18色谱柱(4.6 mm×250 mm,5μm),流动相为乙腈(A)-0.2%磷酸水(B)(梯度洗脱),流速1 mL·min^(-1),检测波长为207、289、325 nm,柱温30℃,所得数据采用主成分分析法(PCA)及偏最小二乘法-判别分析(PLS-DA)处理。结果10种成分在各自线性范围内线性关系良好(r均不低于0.9990),精密度、重复性、稳定性(24 h)试验的RSD均小于3.0%(n=6),平均回收率95.67%~103.99%(n=6),RSD 0.23%~1.98%。10批样品结果显示草珊瑚中黄酮类成分含量叶大于茎,有机酸类成分含量茎大于叶,异嗪皮啶含量茎中最高,PCA和PLS-DA结果显示草珊瑚不同部位可分为3类,变量重要性投影值(variable importance in project,VIP)>1.0筛选出异嗪皮啶、槲皮素-3-O-β-D-葡萄糖醛酸苷、富马酸对区分不同药用部位草珊瑚贡献较大。结论该方法准确可靠,可对草珊瑚的质量控制、临床应用和资源的合理开发利用提供依据。OBJECTIVE To develop an HPLC method for simultaneous determination of ten constituents including fumaric acid,neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid,caffeic acid,isofraxidin,quercetin-3-O-β-D-glucuronide,astilbin,kaempferol-3-O-β-D-glucuronide and rosmarinic acid,in different medicinal parts of Sarcandra glabra,and to compare the differences of 10 constituents by multivariate statistical analysis.METHODS HPLC methods was adopted.The determination was performed on Chromcore AQ C18(4.6 mm×250 mm,5μm)column with mobile phase consisted of acetonitrile(A)-0.2%phosphoric acid(B)(gradient elution)at the flow rate of 1.0 mL·min^(-1),the detection wavelengths was set at 207 nm,289nm and 325 nm.The column temperature maintained at 30℃.The obtained data were processed by principal component analysis(PCA)and partial least squares discriminant analysis(PLS-DA).RESULTS The linearity of the 10 components is good within their linear range(all r>0.999).RSD of precision,reproducibility and stability(24 h)tests were all low than 2.0%(n=6).The average recoveries(n=6)were 95.67%-103.99%,and the RSD was 0.23%-1.98%.The results of 10 batches of samples showed that the content of flavonoids in Sarcandra glabra was higher in the leaves than in the stems,the content of organic acids was higher in the stems than in the leaves,and the content of isofraxidin was the highest in the stems.The results of PCA and PLS-DA showed that different parts of Sarcandra glabra could be divided into three categories,and isofraxidin、quercetin-3-O-β-D-glucuronide and fumaric acid were screened out with variable importance in project(VIP)>1.0,making a great contribution to the differentiation of Sarcandra glabra from different medicinal parts.CONCLUSION The method is accurate and reliable,and can provide evidence for the quality control,clinical application,and scientific resource utilization of Sarcandra glabra.

关 键 词：草珊瑚 高效液相色谱法 含量测定 主成分分析 偏最小二乘法-判别分析 

分 类 号：R284[医药卫生—中药学]