头发甲基苯丙胺质量浓度与胶体金法定性检测结果对比分析研究  

Comparative analysis of methamphetamine mass concentration with qualitative results of colloidal gold method in hhaaiirr ssaammnplleess

作　　者：唐晓欢[1] 吴天辉 孙荣基 顾悦 王鲸[1] 邓媛[1] 彭澎[1] TANG Xiao-huan;WU Tian-hui;SUN Rong-ji;GU Yue;WANG Jing;DENG Yuan;PENG Peng(Yunnan Institute For Drug Abuse,Kunming 650228,China)

机构地区：[1]云南省药物依赖防治研究所,昆明650228

出　　处：《中国药物依赖性杂志》2023年第3期201-205,共5页Chinese Journal of Drug Dependence

摘　　要：目的以甲基苯丙胺质谱检测为阳性的头发样本为研究对象,同时分析甲基苯丙胺质量浓度和胶体金法定性检测结果,探讨二者之间的相互关系。方法头发清洗、烘干后剪碎,精密称取150 mg,甲醇孵育离心,取上层液0.5 ml用滤膜过滤后加入0.5 ml 0.1%的甲酸水进行质谱检测,剩余液体氮气吹干,纯净水复溶,进行胶体金法检测,选取质谱检测为阳性的样本与胶体金法检测结果进行对比分析。结果以胶体金定性结果分为阳性组和阴性组,两组样本的质量浓度均为非正态分布,阳性组占比62.70%,阴性组37.30%,阳性组的质量浓度中位数为3333.3 pg/mg,阴性组的质量浓度中位数为443.6 pg/mg,阳性组的95%可信限下限和阴性组的95%可信限上限在105~1643 pg/mg的范围相互重合,在此浓度范围内的检测结果阳性和阴性均有可能。结论阴性组和阳性组的置信区间大范围重合,无法进行有效区分,提示仅靠胶体金法的定性结果来预测其质量浓度不具备可操作性。Objective e To analyze the relationship between mass concentration of methamphetamine and the qualitative results of colloidal gold method in hair samples that tested positive for methamphetamine mass spectrometry.Methods Hair samples were cut into pieces after cleaned and dried successively,followed by incubation with methanol of 150 mg samples.After centrifuging,0.5ml of the upper liquid was filtered by filter membrane and 0.5ml 0.1%formic acid water was added for mass spectrometry detection.Remaining liquid was dried under nitrogen,dissolved by pure water,and detected by colloidal gold method.The positive samples by mass spectrometry were compared with the results of colloidal gold method.Results Qualitative results of colloidal gold were divided into positive group and negative group.The mass concentration of the two groups were non-normal distribution,the positive group accounted for 62.70%,the negative group 37.30%,the median mass concentration of the positive group was 33333.3 pg/mg,which was 443.6 pg/mg of the negative group.The lower 95%confidence limit of the positive group and the upper 95%confidence limit of the negative group were in the range of 105~1643 pg/mg,and within this concentration range both positive and negative results were possible.ConclusionThe confidence intervals of the negative group and the positive group were largely overlapping,so it was impossible to distinguish them effectively,suggesting that it was not feasible to predict the mass concentration only by the qualitative results of colloidal gold method.

关 键 词：头发 甲基苯丙胺 质量浓度 胶体金法 对比分析 

分 类 号：R917[医药卫生—药物分析学] R749[医药卫生—药学]