氚水对鼠细胞及整体致癌效应的系统评价与Meta分析  

Carcinogenic effect of tritium water on mice or rats:A systematic review and Meta analysis

作　　者：徐凯 李梅[1] 王春平[2] 卢海丽 单文萍 秦秀军[1] XU Kai;LI Mei;WANG Chunping;LU Haili;SHAN Wenping;QIN Xiujun(Shanxi Key Laboratory for Pharmaceutical Toxicology&Radiation Injury Pharmaceuticals/CNNC Key Laboratory for Radiotoxicology and Preclinical Assessment of Radiopharmaceuticals,Department of Radiology and Environmental Medicine,China Institute for Radiation Protection,Taiyuan 030006;School of Public Health,Weifang Medical University,Shandong Weifang 261052;Shanxi Medical University,Taiyuan 030001)

机构地区：[1]中国辐射防护研究院放射医学与环境医学研究所,药物毒理与放射损伤药物山西省重点实验室,中核放射毒理与放射性药物临床前评价重点实验室,太原030006 [2]潍坊医学院公共卫生学院,山东潍坊261052 [3]山西医科大学,太原030001

出　　处：《辐射防护》2023年第4期372-383,共12页Radiation Protection

摘　　要：采用系统评价与Meta分析的方法探讨鼠摄入氚水(HTO)与致癌效应之间的关系,计算机检索PubMed、Embase、The Cochrane Library、Web of Science、中国知网、中国生物医学文献数据库、万方和维普数据库,自数据库建立至2021年2月15日发表的有关鼠摄入氚水后致癌作用的文献。采用Endnote 9.2进行文献管理;应用毒理学数据可靠性评价工具:ToxRTool评价标准进行文献质量评价。结果共纳入10篇实验研究,其中4篇文献报道了体外实验,7篇文献报道体内实验(其中1篇文献体内、体外实验均有报道)。体外实验中,随着细胞受照剂量的增加,细胞生存分数呈指数下降,所拟合直线方程为ln SF=0.047-0.270 D(P<0.001),R^(2)=0.850;以细胞受照剂量为自变量,细胞恶性转化分数(foci/dishes)为因变量拟合方程,方程为y=0.205+0.195x(P<0.001),R^(2)=0.853;剂量反应关系Meta分析显示,随细胞受照剂量的增加,细胞恶性转化风险呈先快速上升后平缓的趋势。体内实验中,腹腔注射途径给药,随着注射药物的放射性活度的增大,中位生存期或平均生存期降低。氚水(HTO)不论对细胞还是对整体动物的致癌效应是确切的,并且导致了生存状况的下降,但极低剂量氚水摄入的致癌效应,还待进一步研究。A systematic review and meta-analysis were used to explore the relationship between exposure to tritium water(HTO)and carcinogenic effect in rats or mice.Database such as PubMed,Embase,The Cochrane Library,Web of Science,CNKI,China Biomedical Literature Database,Wanfang,and VIP were searched with computer.Literature regarding carcinogenicity of rats exposed to tritium water was searched for experimental studies that met the inclusion criteria,with time frame from the establishment of the aformentioned databases to February 15th 2021.Literature was managed by using Endnote 9.2.Toxicological data reliability evaluation tool ToxRTool was used to evaluate the quality of the literature.A total of 10 experimental studies were included.Among them,4 articles reported the malignant transformation experiment in vitro and 7 articles reported the experiment in vivo.For the in Vitro cases,the cell Surviving Fraction decreased exponentially with the increase of radiation dose.The equation was ln SF=0.047-0.270 D(P<0.001),R^(2)=0.850.Taking the radiation dose of cells as the independent variable and the malignant transformation fraction(foci/dishes)as the dependent variable,the fitting equation was y=0.205+0.195x(P<0.001),R^(2)=0.853;Meta-analysis of dose-response relationship showed that the risk of malignant transformation of cells increased rapidly at first and then increased slowly with the increase of radiation dose.For the in Vivo cases,the increase of the activity of intraperitoneal injection resulted in a decrease of median or average survival time.Tritium water(HTO)has a definite carcinogenic effect on both cells and animals and leads to the decline of life quality.However,the carcinogenic effect of very low dose tritium water exposure needs further study.

关 键 词：鼠 氚水 致癌 系统评价 META分析 

分 类 号：Q691.5[生物学—生物物理学]