In vivo spatial-spectral photoacoustic microscopy enabled by optical evanescent wave sensing  

作　　者：Fan Yang Yushu Dong Changjun Min Yejun He Siwei Zhu Hao Liu Wei Song Xiaocong Yuan 

机构地区：[1]Nanophotonics Research Center,Shenzhen Key Laboratory of Micro-Scale Optical Information Technology,Institute of Microscale Optoelectronics,Shenzhen University,Shenzhen,518060,China [2]Research Center for Humanoid Sensing,Zhejiang Laboratory,Hangzhou,311100,China [3]College of Electronics and Information Engineering,Shenzhen University,Shenzhen,518060,China [4]The Institute of Translational Medicine,Tianjin Union Medical Center of Nankai University,Tianjin,300121,China [5]Tianjin Stomatological Hospital,Hospital of Stomatology of Nankai University,Nankai,Tianjin,300041,PR China

出　　处：《Medicine in Novel Technology and Devices》2023年第2期258-266,共9页医学中新技术与新装备（英文）

基　　金：This work was supported in part by the Guangdong Major Project of Basic and Applied Basic Research[grant number 2020B0301030009];the National Natural Science Foundation of China(NSFC)[grant numbers 62175159,62175157,12174204,62071306];the Natural Science Foundation of Guangdong Province,Guangdong,China[2023A1515012888];the Science and Technology Innovation Commission of Shenzhen[grant numbers KQTD20170330110444030,JCYJ20200109113808048,RCJC20210609103232046,JCYJ20200109113601723,JSGG20210802154203011,JSGG20210420091805014,JCYJ20220818101417039];Key Research Project of Zhejiang Lab:K2022MG0AC05;China Postdoctoral Science Foundation:2022M722174.

摘　　要：In addition to offering morphological visualizations via capture of the spatial distributions of optical absorption,photoacoustic imaging technology can reveal abundant physical information about biological particles,including their orientation,density,and viscoelasticity,through analysis of the pressure transients in the spectral domain.However,the low-amplitude wideband photoacoustic signals of intrinsic microscopic optically-absorbing objects under the action of confined photoacoustic excitation power continue to hinder simultaneous photoacoustic structural imaging and spectroscopic analysis of the nonfluorescent chromophores in living biological tissues because of the inadequate responses to photoacoustic impulses observed in most photoacoustic imaging setups that include piezoelectric transducers.Building upon a recently-developed optical evanescent wave sensor that can respond to ultrasound with high sensitivity over a broad frequency range,we propose in vivo spatial-spectral photoacoustic microscopy for recovery of structural imaging in three dimensions and characterization of anatomical features in the acoustic frequency domain.Label-free photoacoustic images of a living zebrafish are acquired in which spectroscopically-resolved differentiation of the microarchitecture is accessed,along with isometric micrometer-scale volumetric visualizations.The proposed imaging technology could potentially provide more comprehensive evaluations of the physiopathological status of living small animals.

关 键 词：Photoacoustic microscopy Optical evanescent wave sensing Spatial-spectral imaging In vivo imaging 

分 类 号：TH742[机械工程—光学工程]