白藜芦醇干预运动性疲劳大鼠心室重构的作用机制  

Action mechanism of resveratrol intervention on ventricular remodeling in exercise-induced fatigue rats

作　　者：章立冰 徐尚 金其贯[1] 胡玉龙[1] Zhang Libing;Xu Shang;Jin Qiguan;Hu Yulong(College of Physical Education,Yangzhou University,Yangzhou 225000,Jiangsu Province,China)

机构地区：[1]扬州大学体育学院,江苏省扬州市225000

出　　处：《中国组织工程研究》2024年第16期2587-2592,共6页Chinese Journal of Tissue Engineering Research

基　　金：2016年度国家重点研发计划项目(2016YFD0400603-02),项目负责人:金其贯。

摘　　要：背景:研究表明白藜芦醇具有缓解运动性疲劳并保护心脏的作用,但其作用机制有待深入研究。目的:探讨白藜芦醇对运动性疲劳大鼠心室重构的保护作用与调控机制。方法:采用随机数字表法将48只雄性SD大鼠分为4组,每组12只:空白对照组大鼠常规饲养;适应性训练1周后,运动性疲劳组和运动性疲劳+白藜芦醇组大鼠进行6周的负荷游泳训练(尾部固定5%体质量铅块,70%-80%最大摄氧量强度),每周6 d,每天60 min,运动后1 h,白藜芦醇组、运动性疲劳+白藜芦醇组灌胃给予50 mg/(kg·d)的白藜芦醇溶液,空白对照组、运动性疲劳组灌胃给予等量的2%二甲基亚砜溶液,每周6 d,每天1次,持续6周。末次干预24 h后,称量大鼠体质量及心脏质量,采用ELISA法检测大鼠血浆肌酸激酶同工酶、心肌肌钙蛋白1及心肌组织中丙酮酸脱氢酶及解偶联蛋白1水平,RT-PCR检测心肌组织心室重构相关因子Foxp1、转化生长因子β1及内皮素1基因表达水平。结果与结论:①与空白对照组比较,运动性疲劳组大鼠体质量减少、心脏质量增加(P<0.05);与运动性疲劳组比较,运动性疲劳+白藜芦醇组大鼠体质量与心脏质量均减少(P<0.05);②与空白对照组比较,运动性疲劳组肌酸激酶同工酶、心肌肌钙蛋白1及解偶联蛋白1水平升高(P<0.01),丙酮酸脱氢酶水平降低(P<0.01);与运动性疲劳组比较,运动性疲劳+白藜芦醇组肌酸激酶同工酶、心肌肌钙蛋白1及解偶联蛋白1水平降低(P<0.05),丙酮酸脱氢酶水平升高(P<0.05);③与空白对照组比较,运动性疲劳组大鼠心肌组织中Foxp1基因表达降低(P<0.01),转化生长因子β1及内皮素1基因表达升高(P<0.01);与运动性疲劳组比较,运动性疲劳+白藜芦醇组大鼠心肌组织中Foxp1基因表达升高(P<0.01),转化生长因子β1及内皮素1基因表达降低(P<0.05);④结果显示,运动性疲劳促使心肌发生适应性改变,造成代偿性肥厚;白藜�BACKGROUND:Studies have shown that resveratrol can relieve exercise-induced fatigue and protect the heart,but its action mechanism needs further study.OBJECTIVE:To explore the protective effect and regulatory mechanism of resveratrol on ventricular remodeling in exercise-induced fatigue rats.METHODS:Totally 48 male Sprague-Dawley rats were randomly divided into four groups,with 12 rats in each group.Rats in the blank control group were fed conventionally.After one week of adaptive training,rats in the exercise-related fatigue group and exercise-related fatigue with resveratrol supplement group were trained by 6-week weight-bearing swimming(5%body mass lead block fixed in the tail,70%-80%maximal oxygen uptake intensity),6 days a week,60 minutes a day.Rats in the resveratrol supplement group and exercise-related fatigue with resveratrol supplement group were given resveratrol(50 mg/kg per day)by gavage one hour after exercise intervention.Blank control group and exercise-related fatigue group were given the same volume of 2%dimethyl sulfoxide,6 days a week,once a day for 6 weeks.The body mass and heart mass of the rats were measured 24 hours after the last intervention.Plasma creatine kinase isoenzyme,cardiac troponin 1,pyruvate dehydrogenase and uncoupling protein 1 levels in myocardial tissue were determined by ELISA.The mRNA expression levels of ventricular remodeling-related factor Foxp1,transforming growth factorβ1 and endothelin 1 were detected by RT-PCR.RESULTS AND CONCLUSION:Compared with the blank control group,the body mass of rats decreased and the heart mass increased in the exercise-related fatigue group(P<0.05).Compared with the exercise-related fatigue group,the body mass and heart mass of the rats reduced in the exercise-related fatigue with resveratrol supplement group(P<0.05).Compared with the blank control group,the levels of creatine kinase isoenzyme,cardiac troponin 1 and uncoupling protein 1 increased(P<0.01),and the level of pyruvate dehydrogenase decreased(P<0.01)in the exercise-related fat

关 键 词：白藜芦醇 运动性疲劳 心肌纤维化 心室重构 心力衰竭 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学] R872.7