深海芽孢杆菌Bacillus megaterium LM-44对亚甲基蓝染料的吸附作用  

The adsorption of methylene blue dye by deep-sea Bacillus megaterium LM-44

作　　者：徐湘薇 张云[1,2,4] 胡云峰 XU Xiangwei;ZHANG Yun;HU Yunfeng(South China Sea Institute of Oceanology,CAS Key Laboratory of Tropical Marine Bio-resources and Ecology,Guangdong Key Laboratory of Marine Materia Medica,Chinese Academy of Sciences,Guangzhou 510301,China;Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou),Guangzhou 511458,China;University of Chinese Academy of Sciences,Beijing 100049,China;Equipment Public Service Center,South China Sea Institute of Oceanology,Chinese Academy of Sciences,Guangzhou 510301,China)

机构地区：[1]中国科学院南海海洋研究所/热带海洋生物资源与生态重点实验室/广东省海洋药物重点实验室,广东广州510301 [2]南方海洋科学与工程广东省实验室(广州),广东广州511458 [3]中国科学院大学,北京100049 [4]中国科学院南海海洋研究所仪器设备公共服务中心,广东广州510301

出　　处：《西北大学学报（自然科学版）》2023年第4期597-607,共11页Journal of Northwest University（Natural Science Edition）

基　　金：国家自然科学基金(21302199);南方海洋科学与工程广东省实验室(广州)人才团队引进重大专项(GML2019ZD0406)。

摘　　要：以深海耐盐菌Bacillus megaterium LM-44作为微生物吸附剂,吸附偶氮染料亚甲基蓝(MB)。利用无机载体硅胶与有机载体大孔树脂对其进行固定化,通过单因素法优化吸附剂反应条件。游离菌株在最适反应条件(吸附剂浓度1.187 mg/mL、溶液pH=9、温度25℃、150 r/min、震荡反应60 min)下,对100 mg/L MB溶液的去除率为70%,吸附容量为50 mg/g,对500 mg/L MB溶液的吸附容量为153 mg/g;硅胶固定化吸附剂的在最适反应条件(吸附剂浓度2 g/L、溶液pH=9、温度25℃、150 r/min、震荡反应120 min)下,对100 mg/L MB溶液的去除率为98%,吸附容量为49 mg/g,对500 mg/L MB溶液的吸附容量为192 mg/g;大孔树脂固定化吸附剂在最适反应条件(吸附剂浓度8 g/L、溶液pH=9、温度25℃、150 r/min、震荡反应180 min)下,对100 mg/L MB溶液的去除率为82%,吸附容量为10 mg/g,对500 mg/L MB溶液的吸附容量为32 mg/g。3种形式的吸附剂在不同无机盐条件下吸附作用较为稳定。Bacillus megaterium LM-44比前期发现的脱色菌Bacillus sp.LM-24具有更高的吸附容量,且对高浓度废水仍然具有较好的去除作用。利用Langmuir与Freundlich吸附等温线模型与动力学函数对吸附过程进行研究,结果表明,3种吸附剂均与准一级动力学拟合度更高,游离菌株吸附作用与Freundlich模型拟合度更高,而固定化吸附剂与Langmuir拟合度更高。Bacillus megaterium LM-44,a deep-sea salt-tolerant bacterium,was used as a microbial adsorbent for the adsorption of the azo dye methylene blue(MB).The inorganic carrier silica gel and organic carrier macroporous resin were used to immobilize it.The reaction conditions of the adsorbent were optimized by the single-factor method,and the optimum reaction conditions for the free strain were the adsorbent concentration of 1.187 mg/mL,the solution pH of 9,the temperature of 25℃,150 r/min,and the removal rate of MB solution of 100 mg/L by shaking reaction for 60 min was 70%,and the adsorption capacity was 153 mg/g when the MB concentration increased to 500 mg/L.The optimum reaction conditions for silica gel immobilized adsorbent were 2 g/L adsorbent concentration,solution pH of 9,temperature 25℃,150 r/min,shaking reaction for 120 min to remove 98%of 100 mg/L MB solution,adsorption capacity of 49 mg/g,adsorption capacity of 500 mg/L MB solution,adsorption capacity of 192 mg/g.The optimum reaction conditions for the immobilized adsorbent of macroporous resin were 8 g/L adsorbent concentration,solution pH of 9,temperature 25℃,150r/min,and 180 min of shaking reaction with 82%removal rate and 10 mg/g adsorption capacity for 100 mg/L MB solution,and 32 mg/g adsorption capacity for 500 mg/L MB solution.Bacillus megaterium LM-44 had a higher adsorption capacity than the previously identified decolorizing bacterium Bacillus sp.LM-24 and still had a better removal of high concentration wastewater.The adsorption process was studied using Langmuir and Freundlich adsorption isotherm models with kinetic functions,and the results showed that all three adsorbents fit better with quasi-level kinetics,and the adsorption of free strains fit better with Freundlich model,while immobilized adsorbents fit better with Langmuir.

关 键 词：深海芽孢杆菌 微生物吸附 亚甲基蓝 高吸附容量 

分 类 号：O647.33[理学—物理化学]