2019—2021年中山市副溶血弧菌食物中毒分离株的病原特征分析  

Etiological characteristics of Vibrio parahaemolyticus strains isolatedfrom foodborne outbreaks in Zhongshan in 2019-2021

作　　者：区金结 李柏生[2] 吴衍恒[1] 郑悦康[1] 吴灿权[1] 仇绮琳 OU Jinjie;LI Baisheng;WU Yanheng;ZHENG Yuekang;WU Canquan;QIU Qilin(Zhongshan Center for Disease Control and Prevention,Zhongshan,Guangdong 528403,China;Guangdong Center for Disease Control and Prevention,Guangzhou,Guangdong 511430,China)

机构地区：[1]广东省中山市疾病预防控制中心,广东中山528403 [2]广东省疾病预防控制中心,广东广州511430

出　　处：《公共卫生与预防医学》2023年第4期72-76,共5页Journal of Public Health and Preventive Medicine

基　　金：中山市副溶血性弧菌毒力基因及多位点序列分型分析(2019J225)。

摘　　要：目的了解中山市2019—2021年副溶血弧菌食物中毒分离株的病原学特征。方法收集中山市2019—2021年8起食物中毒事件分离的37株副溶血弧菌,包含1株剩余食物分离株,36株人源分离株。通过血清学分型、毒力基因检测(tlh、tdh、trh)、药敏试验、脉冲场凝胶电泳(PFGE)以及多位点序列分型(MLST)进行分析本地区副溶血弧菌食物中毒分离株的遗传相关性。结果37株副溶血弧菌共分为4个血清型,分别是O3∶K6、O10∶K4、O4∶K8、O4∶KUT;tdh+、trh-为主要毒力基因型占97.30%(36/37),头孢唑林耐药率较高为40.54%(15株R,22株I),未发现多重耐药菌株。37株副溶血弧菌分为23个PFGE型别,6个聚类群,相关系数在60.4%~100%。共分为9个ST型别,3个复合群,其中以ST3型为主(23株,62.16%)。结论研究发现本市2019—2021年副溶血弧菌食物中毒分离株的优势毒力型为tdh+、trh-,37株代表株可分为6个PFGE聚类群,9个型别,以ST3型为主。此研究发现其罕见血清型O10∶K4,该血清型引起食物中毒的事件有占比增加,提示要加强检测,警惕新的罕见血清型菌株在本地持续流行的风险。Objective To investigate the etiological characteristics of food poisoning isolates of Vibrio parahaemolyticus(VP)from 2019 to 2021 in Zhongshan City.Methods A total of 37 strains of Vibrio parahaemolyticus isolated from 8 food poisoning incidents in Zhongshan City from 2019 to 2021 were collected,including 1 residual food isolate and 36 human isolates.The genetic correlation of Vibrio parahaemolyticus food poisoning isolates in this region was analyzed by serological typing,virulence gene detection(tlh,tdh,and trh),drug sensitivity test,pulsed field gel electrophoresis(PFGE)and multipoint sequence typing(MLST).Results The 37 strains of Vibrio parahaemolyticus were divided into 4 serotypes:O3∶K6,O10∶K4,O4∶K8,and O4∶KUT.The tdh+and trh-were the main virulence genotypes,accounting for 97.30%(36/37).The drug resistance rate of cefazolin was 40.54%(15 strains R,22 strains I),and no multidrug-resistant strains were found.The 37 VP strains were divided into 23 PFGE types and 6 cluster groups,with correlation coefficients ranging from 60.4%-100%.The multipoint sequencing typing showed that the 37 VP strains were divided into 9 ST types and 3 complex groups,of which ST3 type was the main type(23 strains,62.16%).Conclusion This study has found that the dominant virulence types of Vibrio parahaemolyticus food poisoning isolates in Zhongshan City from 2019 to 2021 are tdh+and trh-,and 37 representative strains can be divided into 6 PFGE clusters and 9 ST types with MLST type being mainly ST3.This study has identified the rare serotype O10∶K4 which has caused an increase in the proportion of food poisoning events,suggesting that we should strengthen detection and be alert to the risk of continued local epidemics of new rare serotype strains.

关 键 词：副溶血弧菌 血清型 毒力基因 脉冲场凝胶电泳 多位点序列分型 

分 类 号：R181[医药卫生—流行病学]