氧化石墨烯固定化溶菌酶的研究进展  

Current Research Progress on Application of Graphene Oxide Immobilized Lysozyme

作　　者：李慧[1] 庞姗姗 田家瑶 王云云 薛志飞 龚国利 LI Hui;PANG Shan-shan;TIAN Jia-yao;WANG Yun-yun;XUE Zhi-fei;GONG Guo-li(College of Art and Design,Shaanxi University of Science&Technology,Shaanxi Xi an 710021;College of Food and Bioengineering,Shaanxi University of Science&Technology,Shaanxi Xi an 710021,China)

机构地区：[1]陕西科技大学设计与艺术学院,陕西西安710021 [2]陕西科技大学食品与生物工程学院,陕西西安710021

出　　处：《广州化工》2023年第8期16-19,共4页GuangZhou Chemical Industry

基　　金：陕西省重点研发计划项目(2019NY-194);陕西省西安市未央区科技计划项目(202038)。

摘　　要：氧化石墨烯(GO)具有高比表面积、丰富的活性位点、良好的亲水性以及生物相容性,是一种酶固定化的良好载体。综述了近年来GO在溶菌酶固定化方面的研究成果,介绍了GO固定化溶菌酶的两种途径;解析了GO对固定化溶菌酶的吸附量及酶活性的影响;阐述了调控酶与载体间的相互作用、避开酶活性位点、减少对酶构象的变化,避免酶在固定过程中活性的损失的方法。最后指出,改性GO载体和改良固定方法,是未来GO固定化溶菌酶研究的主要方向。Immobilized enzyme technology can improve the stability of the free enzyme,easy to separate and reuse,and reduce production costs.Graphene oxide(GO)is an excellent carrier for enzyme immobilization with high specific surface area,abundant active sites,good hydrophilicity,and biocompatibility.Two ways of lysozyme immobilization by GO were summarized.The reason for the decrease of lysozyme activity caused by GO fixation was analyzed,and the effect of GO on the adsorption capacity and activity of immobilized lysozyme was parsed.Furthermore,the methods of controlling the interaction between enzyme and carrier,avoiding the active site of the enzyme,reducing the change of conformation of the enzyme,and avoiding the loss of enzyme activity during immobilization were reviewed.Finally,it was pointed out that modified GO carriers and improved immobilization methods were the main directions for future research on GO immobilized lysozyme.

关 键 词：氧化石墨烯 溶菌酶 酶活 修饰 吸附量 

分 类 号：Q814.2[生物学—生物工程]