壮药三七姜不同提取部位抗炎镇痛活性研究  

Study on the anti-inflammatory and analgesic activities of different extraction parts of Zhuang medicine Sanqi Ginger

作　　者：黄团心 刘星晨 梁洁[2,4] 郑飘雪 梁炎丽 陆春莲 HUANG Tuanxin;LIU Xingchen;LIANG Jie;ZHENG Piaoxue;LIANG Yanli;LU Chunlian(Guangxi Institute for Food and Drug Control,Nanning Guangxi 530021;Guangxi University of Chinese Medicine,Nanning Guangxi 530200;Guangxi UniversityKey Laboratory of Extraction,Purification and Quality Analysis of Tra-ditional Chinese Medicine,Nanning Guangxi 530200;South China Branch of National Engineering Research Center for Manufacturing Technology of Traditional Chinese Medicine Solid Preparation,Nanning Guangxi 530200)

机构地区：[1]广西壮族自治区食品药品检验所,广西南宁530021 [2]广西中医药大学,广西南宁530200 [3]广西高校中药提取纯化与质量分析重点实验室,广西南宁530200 [4]中药固体制剂制造技术国家工程研究中心华南分中心,广西南宁530200

出　　处：《广西中医药》2023年第3期56-60,共5页Guangxi Journal of Traditional Chinese Medicine

基　　金：广西自然科学基金项目(编号:2020GXNSFAA238026);广西壮瑶药重点实验室项目(编号:GXZYZZ2020-05);广西一流学科建设项目(桂教科研〔2018〕12号);广西科技基地和人才专项(桂科AD20238058);广西壮瑶药协同创新中心(桂教科研〔2013〕20号);广西壮瑶药重点实验室(桂科基字〔2014〕32号);广西壮族自治区民族药资源与应用工程研究中心(桂发改高技函〔2020〕2605号);广西中医药重点学科壮药学(编号:GZXK-Z-20-64);第八批广西壮族自治区特聘专家项目(桂人才通字〔2019〕13号);广西高等学校千名中青年骨干教师培育计划(桂教人〔2019〕5号)。

摘　　要：目的:研究壮药三七姜的抗炎镇痛作用及筛选其药理活性部位。方法:建立二甲苯致小鼠耳廓肿胀、小鼠棉球肉芽肿、醋酸致小鼠腹腔毛细血管通透性亢进、小鼠热板致痛及醋酸致小鼠扭体模型,筛选三七姜抗炎镇痛药理活性部位。结果:与模型组比较,在抗炎实验中,三七姜石油醚高、中剂量组和乙酸乙酯高剂量组可明显抑制小鼠耳廓肿胀程度,抑制棉球所致的小鼠肉芽组织增生,降低醋酸致小鼠毛细血管通透性(P<0.05或P<0.01);在镇痛实验中,三七姜石油醚高、中剂量组和乙酸乙酯高剂量组能减轻热板刺激引起的疼痛,提高痛阈值,减少醋酸引起的小鼠疼痛的扭体次数(P<0.05或P<0.01)。结论:壮药三七姜有抗炎镇痛作用,其药理活性部位为石油醚部位和乙酸乙酯部位。Objective:To investigate the anti-inflammatory and analgesic effects of different extracts of Sanqi Ginger and screen its pharmacologically active parts.Methods:Xylene-induced mouse ear swelling and mouse cotton ball granuloma,acetic acid-induced increase in mouse abdominal capillary permeability,plate-induced pain,and ace⁃tic acid-induced mouse writhing model were established to screen the pharmacological active parts of Sanqi Ginger.Re⁃sults:Compared with the model group,in the anti-inflammatory experiment,the high and medium doses of Sanqi Gin⁃ger petroleum ether and the high dose of ethyl acetate could significantly inhibit the degree of mouse ear swelling,inhib⁃it the proliferation of mouse granulation tissue caused by cotton balls,and reduce the capillary permeability induced by acetic acid(P<0.05 or P<0.01);in the analgesic experiment,the high and medium doses of Sanqi Ginger petroleum ether and the high dose of ethyl acetate could reduce the pain caused by hot plate stimulation,increase the pain thresh⁃old,and reduce the number of mouse writhing caused by acetic acid-induced pain(P<0.05 or P<0.01).Conclusion:Zhuang medicine Sanqi Ginger has function of anti-inflammatory and analgesic effect,and its pharmacologically active parts are petroleum ether and ethyl acetate parts.

关 键 词：三七姜 抗炎 镇痛 活性部位 

分 类 号：R285.5[医药卫生—中药学]