富含羟基酪醇橄榄油对衰老小鼠的影响  

Effect of olive oil containing hydroxyl tyrosol on aging mice

作　　者：陈文东[1] 叶文斌[1] 朱秀娟[1] 王昱[1] 黄新异[2] 邸多隆[2] CHEN Wendong;YE Wenbin;ZHU Xiujuan;WANG Yu;HUANG Xinyi;DI Duolong(School of Agriculture and Forestry Technology,Longnan Teachers College,Chengxian 742500,China;Key Laboratory of Chemistry of North Western Plant Resources&Key Laboratory for Natural Medicine of Gansu Province,Lanzhou Institute of Chemical Physics,Chinese Academy of Sciences,Lanzhou 730000,China)

机构地区：[1]陇南师范高等专科学校农林技术学院,甘肃成县742500 [2]中国科学院兰州化学物理研究所中科院西北特色植物资源化学重点实验室和甘肃省天然药物重点实验室,甘肃兰州730000

出　　处：《甘肃农业大学学报》2023年第3期15-22,32,共9页Journal of Gansu Agricultural University

基　　金：2021年甘肃省科技重大专项(21ZD4NK045);2022年甘肃省重点研发计划项目(22YF7FK220);2021年甘肃省自然科学基金项目(21JR7RK912);2023年甘肃省高校教师创新基金项目(2023B-412);陇南市科技计划项目(2021-11)。

摘　　要：【目的】为研究富含羟基酪醇橄榄油(Olive oil rich in hydroxyl tyrosol,OHT)对衰老小鼠的影响。【方法】利用小鼠颈部皮下注射500 mg/kg的D-半乳糖(D-galactose)溶液构建亚急性衰老模型,建模成功后,将小鼠随机分成7组,分别为空白对照组(CK_(0))、模型组(Model group)、维生素E阳性对照组(VE positive control group)、普通橄榄油组(Cormal olive oil group)和OHT高、中、低剂量(200、100、50 mg/kg)处理组(OHT high,medium,low-dose group),连续灌胃28 d,检测小鼠体质量变化及脏器指数、血清中谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)、超氧化物歧化酶(superoxide dismutase,SOD)、过氧化氢酶(catalase,CAT)活性及丙二醛(malondialdehyde,MDA)含量的变化,观察肝组织形态学变化。【结果】OHT使衰老小鼠的体质量和脏器指数显著升高(P<0.05,P<0.01),肝脏病理变化得到改善,血清CAT、GSH-Px和SOD活性显著升高(P<0.05,P<0.01),MDA含量显著降低(P<0.05,P<0.01),以上指标变化在OHT高剂量组尤为明显,且OHT干预效果强于普通橄榄油。【结论】OHT能提高机体抗氧化能力,进而发挥抗衰老的作用。【Objective】To study the effects of olive oil containing hydroxyl tyrosol(OHT)on aging mice.【Method】An aging animal model was established by subcutaneous injection of 500 mg/kg Dgalactose in the neck,and after successful modeling,the mice were randomly divided into 7 groups:blank control group,model group,VE positive control group,common olive oil group,OHT high,medium and low dose treated groups(200 mg/kg,100 mg/kg,50 mg/kg),the OHT was administered to the mice by intragastric administration for 28 consecutive days.The weight and viscera indexes of each group were calculated,and the activities of glutathione peroxidase(GSH-Px),superoxide dismutase(SOD),catalase(CAT)and malondialdehyde(MDA)in the serum were determined,and the morphology changes of the liver tissue were observed.【Result】OHT could significantly improve the body weight and organ index in aging mice(P<0.05,P<0.01),ameliorate the pathological changes of the liver,significantly increase the activity of serum CAT,GSH-Px and SOD(P<0.05,P<0.01),and significantly reduced MDA content(P<0.05,P<0.01).The changes in the above indexes were particularly evident in the high-dose OHT group,and the intervention effect of OHT was stronger than that of normal olive oil.【Conclusion】OHT could improve the body’s antioxidant capacity to play an anti-aging role.

关 键 词：羟基酪醇 橄榄油 衰老小鼠 抗氧化 抗衰老 

分 类 号：TS225.19[轻工技术与工程—粮食、油脂及植物蛋白工程]