Elevated and atmospheric-level methane consumption by soil methanotrophs of three grasslands in China  

作　　者：Yufang Wang Yuanfeng Cai Fujiang Hou Saman Bowatte Zhongjun Jia 

机构地区：[1]State Key Laboratory of Herbage Improvement and Grassland Agroecosystems,Key Laboratory of Grassland Livestock Industry Innovation,Ministry of Agriculture and Rural Affairs,College of Pastoral Agriculture Science and Technology,Lanzhou University,Lanzhou,Gansu Province,China [2]State Key Laboratory of Soil and Sustainable Agriculture,Institute of Soil Science,Chinese Academy of Sciences,Nanjing,Jiangsu Province,China [3]AgResearch Limited,Grasslands Research Centre,Palmerston North,New Zealand [4]Northeast Institute of Geography and Agroecology,Chinese Academy of Sciences,Changchun,Jilin Province,China

出　　处：《Grassland Research》2023年第2期85-96,共12页草地研究（英文）

基　　金：National Natural Science Foundation of China,Grant/Award Numbers:42277114,91751204,41877062。

摘　　要：Background:Methane(CH4)oxidation driven by soil aerobic methanotrophs demonstrates the capacity of grassland as a CH4 sink.Methods:In this study,we compared the oxidation characteristics of atmospheric-level and elevated concentration(10%)CH4 in a typical grassland(steppe)on the Loess Plateau,an alpine meadow(meadow)on the Qinghai-Tibet Plateau,and an inland arid-area artificial grassland(pasture)in northwest China and investigated the communities of active methanotrophs and their contribution to CH4 oxidation using DNA-based stable-isotope probing and Illumina Miseq sequencing.Results:The results showed that the oxidation of atmospheric CH4 only occurred in steppe and meadow soils where the USCγgroup of methanotrophs was numerically dominant in the methanotroph community.Pasture soils,with their very low relative abundance of USCγ,did not show atmospheric CH4 oxidation.However,a DNA-stable isotope probing experiment with 10%CH4 indicated that conventional CH4 oxidizers(Methylocaldum and Methylocystis)rather than USCγcommunities assimilated significant amounts of 13CH4 for growth.Conclusions:The CH4 oxidation mechanisms in the three experimental grassland soils varied significantly.The USCγgroup may be obligate oligotrophic microorganisms or their growth requires specific unknown conditions.

关 键 词：grasslands methane oxidation METHANOTROPHS uncultivated methanotrophs USCγ 

分 类 号：S812.2[农业科学—草业科学]