老鹳草化学成分及抗肿瘤活性研究  

Chemical Components and Antitumor Activity of Geranium wilfordii Maxim.

作　　者：孔楚楚 柳志诚 叶丹阳 张园园[1] 刘永刚[1] KONG Chu-chu;LIU Zhi-cheng;YE Dan-yang;ZHANG Yuan-yuan;LIU Yong-gang(School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 100029,China)

机构地区：[1]北京中医药大学中药学院,北京100029

出　　处：《中国现代中药》2023年第6期1187-1193,共7页Modern Chinese Medicine

基　　金：国家重点研发计划项目(2018YFC1706505)。

摘　　要：目的:对牻牛儿苗科老鹳草属植物老鹳草Geranium wilfordii Maxim.干燥地上部分的化学成分进行研究,并对分离出的化学成分进行体外抗肿瘤活性评价。方法:采用硅胶柱色谱、制备薄层色谱等对老鹳草的75%乙醇提取物进行分离纯化,并通过核磁共振波谱法对化合物进行结构鉴定。采用人肝癌细胞HepG2,通过CCK-8实验检测分离纯化得到的化合物的体外抗肿瘤活性。结果:从老鹳草的75%乙醇提取物中共分离纯化得到17个化合物,分别鉴定为水杨酸(1)、没食子酸甲酯(2)、没食子酸乙酯(3)、1,2,4-苯三酚(4)、原儿茶酸(5)、(6S,9R)-6,9-二羟基-3-酮基-α-香堇醇(6)、短叶苏木酚酸乙酯(7)、没食子酸(8)、euphorhirtin G (9)、异柯里拉京(10)、金丝桃苷(11)、槲皮素(12)、烟酰胺(13)、山柰酚(14)、短叶苏木酚(15)、莽草酸(16)、槲皮素-3-O-(6″-没食子酰基)-β-D-半乳糖苷(17)。化合物2~4、7、8、10、14、15、17具有体外抗HepG2细胞增殖的活性[半数抑制浓度(IC50)<300μmol·L^(–1)]。结论:化合物2、4、6、9、10、13、16、17均为首次从该植物中分离得到,化合物4、6、9、10、13为首次从老鹳草属植物中分离得到。化合物10、14体外抗HepG2细胞增殖活性较好,IC50分别为27.95、18.24μmol·L^(–1)。Objective:To explore the chemical components on the dry ground part of Geranium wilfordii Maxim.,and conduct research on in vitro antitumor activity of the isolated chemical components.Methods:The 75%ethanol extract of G.wilfordii Maxim.was separated and purified by silica gel column chromatography and preparation of thin-layer chromatography,and the chemical structures of the compounds were identified by nuclear magnetic resonance spectroscopy(NMRS).The human hepatoma cell HepG2 was employed to detect the in vitro antitumor activity of these compounds by CCK-8 experiments.Results:A total of 17 compounds were isolated and purified from the 75%ethanol extract of G.wilfordii Maxim.The compounds were identified as salicylic acid(1),methyl gallate(2),ethyl gallate(3),1,2,4-phenylenetriol(4),protocatechuic acid(5),(6S,9R)-6,9-dihydroxy-3-oxo-α-ionol(6),ethyl brevifolin carboxylate(7),gallic acid(8),euphorhirtin G(9),isocorilagin(10),hyperoside(11),quercetin(12),nicotinamide(13),kaempferol(14),brevifolin(15),shikimic acid(16),quercetin-3-O-β-D-(6″-galloyl)-galactopyranoside(17).There were eight compounds with in vitro antitumor activity(IC50<300μmol·L^(–1)),including compounds 2-4,7,8,10,14,15,and 17.Conclusion:Compounds 2,4,6,9,10,13,16,and 17 are isolated from this plant for the first time,and compounds 4,6,9,10,and 13 are isolated for the first time from Geranium.Compounds 10 and 14 have good in vitro antitumor activity,with IC50 of 27.95,18.24μmol·L^(–1).

关 键 词：老鹳草 化学成分 抗肿瘤 鞣质 黄酮 

分 类 号：R284[医药卫生—中药学]