含“巴沙嘎”类(瞿麦)药材方剂的数据挖掘及其治疗肝病的作用机制分析  

Bashaga-containing Prescriptions:Data Mining of Key Medicinal Materials and Mechanism in Treatment of Liver Diseases

作　　者：孟祥玺 周保昌 刘一波 吴昊[3] 高原 郭文芳 卢怀民[2] 曹晓东[3] 李旻辉 MENG Xiang-xi;ZHOU Bao-chang;LIU Yi-bo;WU Hao;GAO Yuan;GUO Wen-fang;LU Huai-min;CAO Xiao-dong;LI Min-hui(Inner Mongolia Hospital of Traditional Chinese Medicine,Hohhot 010020,China;Baotou Medical College,Baotou 014040,China;Inner Mongolia Medical University,Hohhot 010110,China;Inner Mongolia Institute of Traditional Chinese and Mongolian Medicine,Hohhot 010010,China;Songshan Traditional Chinese and Mongolian Medicine Hospital,Chifeng 024046,China)

机构地区：[1]内蒙古自治区中医医院,内蒙古呼和浩特010020 [2]包头医学院,内蒙古包头014040 [3]内蒙古医科大学,内蒙古呼和浩特010110 [4]内蒙古自治区中蒙医药研究院,内蒙古呼和浩特010010 [5]赤峰松山中医蒙医医院,内蒙古赤峰024046

出　　处：《中国现代中药》2023年第6期1266-1279,共14页Modern Chinese Medicine

基　　金：国家重点研发计划项目(2021YFE0190100);国家自然科学基金项目(81874336);中央本级重大增减支项目(2060302);包头医学院“花蕾计划”项目(HL2021008)。

摘　　要：目的:挖掘含“巴沙嘎”类药材治疗肝病的核心药物,实验验证核心药物治疗肝炎的作用机制。方法:通过数据挖掘技术挖掘出蒙古族医(以下简称蒙医)应用“巴沙嘎”类药材治疗肝病的核心药物,并利用四氯化碳诱导的肝损伤动物实验、网络药理学、分子对接技术3种方法,分析核心药物发挥治疗作用的机制。结果:数据挖掘技术挖掘含“巴沙嘎”类药材治疗的肝病方剂共46首,涉及蒙古族药143味,用药总频数达585次,其中应用的“巴沙嘎”类药材全部都为瞿麦,与瞿麦配伍的高频药物(≥5次)为红花(41次,89.13%)、五灵脂(36次,78.26%)、牛黄(25次,54.34%),挖掘出核心药物“瞿麦-红花”。“瞿麦-红花”可以降低肝损伤小鼠模型血清中丙氨酸转氨酶、天冬氨酸转氨酶的水平,减轻实验小鼠肝细胞中炎性因子浸润,能够减轻模型小鼠肝细胞坏死和细胞水肿的程度。网络药理学筛选出“瞿麦-红花”潜在活性成分36个,治疗肝炎作用靶点120个。其中蛋白激酶B1(Akt1)、白细胞介素-6(IL-6)、肿瘤蛋白p53(TP53)、血管内皮生长因子A(VEGFA)、肿瘤坏死因子(TNF)、丝裂原活化蛋白激酶(MAPK)-8、JUN原癌基因(JUN)、胱天蛋白酶(CASP)-3、MAPK1、基质金属肽酶(MMP)-9为“瞿麦-红花”治疗肝炎的关键靶点。与TNF、PI3K/Akt和HIF-1信号通路有关,主要为调节体内氧化应激及凋亡,减轻炎症的发生等。结论:蒙医应用“巴沙嘎”类药材治疗肝病的核心药物为“瞿麦-红花”,治疗肝炎通过调节体内氧化应激及凋亡,调控TNF、磷脂酰肌醇3-激酶/蛋白激酶B(PI3K/Akt)和缺氧诱导因子(HIF)-1信号通路,调控炎症因子,从多个角度减少肝脏炎症的发生,从而达到保护肝脏的目的。Objective:To explore the core medicinal materials in Bashaga-containing prescriptions for treating liver diseases and experimentally verify the mechanism of the core medicinal materials in treating hepatitis.Methods:The core medicinal materials in Bashaga-containing prescriptions for treating liver diseases were identified by data mining,and their mechanisms in exerting the therapeutic effects were analyzed by animal experiments on carbon tetrachloride(CCl4)-induced liver injury,network pharmacology,and molecular docking.Results:A total of 46 prescriptions containing Bashaga for liver diseases were screened out,involving 143 Mongolian medicinal materials,with the total frequency of 585 times.Bashaga in all the prescriptions was Dianthi Herba.The high-frequency medicinal materials(≥5 times)used in combination with Dianthi Herba were Carthami Flos(41 times,89.13%),Faeces Trogopterori(36 times,78.26%),and Bovis Calculus(25 times,54.34%),and the core pair of Dianthi Herba-Carthami Flos was discovered.Dianthi Herba-Carthami Flos can lower the levels of alanine aminotransferase and aspartate aminotransferase in the serum of the mouse model of liver injury,and reduce the infiltration of inflammatory cytokines in the liver cells,and alleviate hepatocyte necrosis and cellular edema in the model mice.The network pharmacology identified 36 potentially active ingredients of Dianthi Herba-Carthami Flos and 120 targets for the treatment of hepatitis.Protein kinase(Akt1),interleukin-6(IL-6),tumor protein p53(TP53),vascular endothelial growth factor A(VEGFA),tumor necrosis factor(TNF),mitogen-activated protein kinase 8(MAPK8),JUN proto-oncogene(JUN),cysteine aspartate-specific protease 3(CASP3),MAPK1,and matrix metalloproteinase 9(MMP9)were the key targets of Dianthi Herba-Carthami Flos for treating hepatitis.TNF,phosphatidylinositol 3-kinase(PI3K)/Akt,and hypoxiainducible factor-1(HIF-1)signaling pathways were involved in the regulation of oxidative stress and apoptosis in vivo and the reduction of inflammation.Conclusion:Dia

关 键 词：巴沙嘎 肝病 数据挖掘 实验验证 网络药理学 

分 类 号：R282[医药卫生—中药学] R285.5[医药卫生—中医学]