白薇饮片及女金丸的掺伪鉴别  

Identification of Adulterated Cynanchi Atrati Radix et Rhizoma Decoction Pieces and Nvjin Pill Preparations

作　　者：穆向荣 汪冰 林林 郭东晓 于凤蕊 唐真真 孟德新 林永强 MU Xiang-rong;WANG Bing;LIN Lin;GUO Dong-xiao;YU Feng-rui;TANG Zhen-zhen;MENG De-xin;LIN Yong-qiang(Shandong Provincial Institute for Food and Drug Control,National Medical Products Administration Key Laboratory for Quality Evaluation of Gelatin Products,Shandong Engineering Laboratory for Standard Innovation and Quality Evaluation of TCM,Shangdong Engineering Research Center of Generic Technologies for Traditional Chinese Medicine Formula Granules,Jinan 250101,China;Shuyu Civilian Pharmacy,Jinan 250000,China;Zhangqiu Hospital of Traditional Chinese Medicine,Jinan 250200,China)

机构地区：[1]山东省食品药品检验研究院国家药品监督管理局胶类产品质量评价重点实验室/山东省中药标准创新与质量评价工程实验室/中药配方颗粒共性技术山东省工程研究中心,山东济南250101 [2]漱玉平民大药房,山东济南250000 [3]章丘中医院,山东济南250200

出　　处：《中国现代中药》2023年第6期1319-1325,共7页Modern Chinese Medicine

基　　金：山东省重点研发计划(重大科技创新工程)项目(2021CXGC010511);山东省人文社会科学课题(2021-YYGL-44);泉城产业领军人才支持计划创新团队项目(MRJT2105);“新高校20”项目(202228096)。

摘　　要：目的:解决白薇饮片及含白薇的制剂女金丸中白薇掺伪的问题。方法:采用性状鉴别、显微鉴别和化学分析方法研究白薇饮片及女金丸中白薇掺伪的问题。结果:按照《中华人民共和国药典》 2020年版白薇项下的性状和显微鉴别方法难以区分白薇及其伪品老瓜头,但两者的薄层色谱和高效液相色谱存在明显差异,进一步采用高效液相色谱-离子阱-飞行时间质谱联用仪确认特征色谱峰为β-D-呋喃果糖基-(2→1)-α-D-[6-O-芥子酰基]-吡喃葡萄糖苷。结论:建立的检查方法操作简单、灵敏度高,可为白薇饮片及含白薇制剂的质量提升提供参考。Objective:To resolve the quality problem of adulterated Cynanchi Atrati Radix et Rhizoma decoction pieces and Nvjin Pill Preparations.Methods:The morphological,microscopic,and chemical characteristics of Cynanchi Atrati Radix et Rhizoma were taken as references for distinguishing between Cynanchi Atrati Radix et Rhizoma and its adulterant.Result:According to the morphological and microscopic characteristics recorded in the Chinese Pharmacopoeia(2020 edition),we found it difficult to distinguish Cynanchi Atrati Radix et Rhizoma from its adulterant Cynanchum komarovii.However,the thin-layer chromatography(TLC)and high performance liquid chromatography(HPLC)revealed significant differences between Cynanchi Atrati Radix et Rhizoma and its adulterant.HPLC-ion trap-time of flight mass spectrometry demonstrated thatβ-D-fructofuranosyl-(2→1)-α-D-[6-O-sinapoyl]-glucopyranoside was the characteristic component.Conclusion:The established identification method demonstrates simple operation and high sensitivity,providing technical support for the quality control of Cynanchi Atrati Radix et Rhizoma decoction pieces and Nvjin Pill Preparations.

关 键 词：白薇 女金丸 老瓜头 β-D-呋喃果糖基-(2→1)-α-D-[6-O-芥子酰基]-吡喃葡萄糖苷 

分 类 号：R286[医药卫生—中药学]