大负荷运动后骨骼肌核心时钟基因Bmal1/Clock、Cry1/Per1的节律性振荡变化趋势  

Influence of Heavy Exercise on Rhythmic Oscillation Trend of Skeletal Muscle Core Clock Genes Bmal1/Clock and Cry1/Per1

作　　者：丁海丽[1] 傅泽铤 夏雨 DING Haili;FU Zeting;XIA Yu(Chengdu Sport University,Chengdu 610041,China)

机构地区：[1]成都体育学院,四川成都610041

出　　处：《中国体育科技》2023年第7期90-96,F0003,共8页China Sport Science and Technology

基　　金：国家重点研发计划“科技冬奥”重点专项(2018YFF0300604);国家自然科学基金资助项目(81904318);运动医学四川省重点实验室暨国家体育总局运动医学重点实验室资助项目(2021-A021)。

摘　　要：目的:探讨大负荷运动对骨骼肌核心时钟基因节律性表达的影响。方法:将208只8周龄SD大鼠随机分为对照(control,C)组与运动(Exercise,E)组,E组予以一次大负荷运动训练。每6 h取各组比目鱼肌,采用透射电镜观察骨骼肌纤维超微结构,通过实时荧光定量PCR实验测定骨骼肌核心时钟基因Bmal1、Clock、Cry1、Per1表达量,并使用余弦分析软件circacompare(R package)获取拟合余弦曲线参数,分析其节律性振荡的变化趋势。结果:1)C组Bmal1、Clock、Cry1、Per1 mRNA在72 h内呈现3个完整近日节律周期;E组Bmal1 mRNA、Cry1和Per1mRNA在0~24 h、Clock mRNA在0~72 h近日节律消失。2)与C组相比,E组Bmal1 mRNA在0、6、12和18 h显著升高,Clock mRNA在0 h时显著升高;Cry1、Per1 mRNA在0、12 h显著降低。3)C组各时相肌纤维超微结构正常,E组0、24和48 h均出现不同程度肌小节结构破坏、线粒体肿胀及聚集等改变,72 h有所缓解。结论:一次大负荷运动可诱发骨骼肌核心时钟基因Bmal1/Clock、Cry1/Per1节律振荡紊乱,其变化趋势与肌纤维超微结构损伤时相基本一致。Objective:To investigate the effect of heavy exercise on the rhythmic oscillation of skeletal muscle core clock genes.Methods:Two hundred and eight 8-week-old SD rats were randomly divided into the control(C)group and the exercise(E)group.The rats in group E were trained with heavy exercise for one time,and the soleus muscles were taken to detect indices every 6 hours.The ultrastructure of skeletal muscle fibers was observed by transmission electron microscope.The expression of skeletal muscle core clock genes,Bmal1,Clock,Cry1 and Per1 were measured by real-time fluorescence quantitative PCR.The parameters of fitting cosine curve were obtained by cosine analysis software circacompare(R package),and the change trend of rhythmic oscillation was analyzed.Results:1)In group C,three complete circadian rhythm cycles were observed of Bmal1,Clock,Cry1 and Per1 mRNA within 72 hours.In group E,the circadian rhythm of Bmal1 mRNA,Cry1 and Per1 mRNA disappeared at 0-24 h,Clock mRNA disappeared at 0-72 h.2)Compared with group C,Bmal1 mRNA in group E increased significantly at 0,6,12 and 18 h,and Clock mRNA increased significantly at 0 h;Cry1,Per1 mRNA decreased significantly at 0 h and 12 h.3)The ultrastructure of muscle fibers in group C was normal at all timepoints.In group E,changes,such as structural destruction of sarcomere,swelling and aggregation of mitochondria,occurred at 0,24 and 48 h,and relieved at 72 h.Conclusions:Heavy exercise could induce the rhythm oscillation disorder of Bmal1/Clock and Cry1/Per1 in skeletal muscle,which tendency was consistent with the ultrastructural damage of skeletal muscle fibers.

关 键 词：骨骼肌 时钟基因 Bmal1/Clock Cry1/Per1 生物节律 生物钟 

分 类 号：G804.2[文化科学—运动人体科学]