Ethanol extract of Annona muricata Linn fruit perform antidiabetic effect on type 2 diabetic mice through α-glucosidase inhibition  

作　　者：QIN Hai-long YAO Jia-hui WANG Yan XU Qi-peng LIU Zheng LI You-bin GONG Jing-wen 

机构地区：[1]Hainan Provincial Key Laboratory for Research and Development of Tropical Herbs,Key Laboratory of Tropical Translational Medicine of Ministry of Education,Haikou Key Laboratory of Li Nationality Medicine,School of Pharmacy,Hainan Medical University,Haikou 571100,China

出　　处：《Journal of Hainan Medical University》2023年第7期1-7,共7页海南医学院学报（英文版）

基　　金：supported by 2020 College Students Innovation and Entrepreneurship Training Program(X202011810069);the National Natural Science Foundation of China(81460591)。

摘　　要：Objective:To explore the anti-diabetic effects and its underlying mechanism of Annona muricata Linn fruit ethanol extract(AME).Methods:Streptozotocin-induced type 2 diabetic(T2DM)mouse model was constructed.Those diabetic mice were randomly grouped and given 50 mg/kg acarbose or AME(200 mg/kg,100 mg/kg or 50 mg/kg)for four weeks.The body weight,postprandial blood glucose and glycosylated hemoglobin levels were measured during the administration.After the administration,a glucose tolerance test was performed,and the levels of triglycerides,cholesterol and low-density lipoproteins in mice were detected by biochemical test kits.The inhibitory activity of AME onα-glucosidase in vivo and in vitro was determined by enzyme inhibition tests.Results:AME significantly reduced weight gain,postprandial blood glucose,glycosylated hemoglobin and low-density lipoprotein levels in T2DM mice;enhanced glucose tolerance and pancreaticβ-cell function of T2DM mice;inhibitedα-glucosidase activity in mouse intestine in an noncompetitive manner.Conclusion:AME may noncompetitive inhibitα-glucosidase activity and reduce postprandial glucose intake to achieve a therapeutic and regulatory effect on type 2 diabetes.

关 键 词：Annona muricata Linn Type 2 diabetes mellitus Α-GLUCOSIDASE Postprandial blood glucose 

分 类 号：R587.1[医药卫生—内分泌]