Oyster studies reveal the duplication and functional diversification of Bivalvia caspase-8 genes  

作　　者：Shaoxi DENG Tao QU Guofan ZHANG Fei XU 

机构地区：[1]CAS and Shandong Province Key Laboratory of Experimental Marine Biology,Center for Ocean Mega-Science,Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,China [2]University of Chinese Academy of Sciences,Beijing 100049,China [3]Laboratory for Marine Biology and Biotechnology,Qingdao National Laboratory for Marine Science and Technology,Qingdao 266000,China [4]NHC Key Laboratory of Diagnosis and Therapy of Gastrointestinal Tumor,Gansu Provincial Hospital,Lanzhou 730000,China

出　　处：《Journal of Oceanology and Limnology》2023年第3期1109-1121,共13页海洋湖沼学报（英文）

基　　金：Supported by the Science&Technology Innovation Project of Laoshan Laboratory (No. LSKJ202203001);the Center for Ocean Mega-Research of Science;Chinese Academy of Sciences (No. COMS2019Q11);the GHfund B (No. 20210702);the Taishan Scholars Program

摘　　要：Caspase-8,first classified as a pro-apoptotic caspase,is considered to have arisen from duplication with caspase-10 and involves multiple immune and inflammatory responses in mammals.However,few are known on the phylogeny and function of caspase-8 in molluscs,one of the largest phyla in marine invertebrates.In this study,we conducted phylogenetic and functional analysis on molluscan caspase-8-like genes.Results indicate that duplication occurred in molluscan caspase-8-like genes,resulting in at least two caspase-8 copies in some groups of bivalves.Additional studies in Pacific oyster Crassostrea gigas showed different spatio-temporal expression patterns and subcellular localizations of CgCaspase-8-1 and CgCaspase-8-2.While no interaction was observed between CgCaspase-8-2 and CgFADD,the adaptor molecule in apoptosis,yeast two-hybrid and co-immunoprecipitation assays suggested the interaction between CgCaspase-8-1 and CgFADD,indicating its pro-apoptotic function.In addition,CgCaspase-8-1 showed interaction with the CARD domain of CgRIG-I.Together with two NF-κB subunits(Cgp105 and CgRel),their transcripts were up-regulated in response to poly(I:C)stimuli,supporting the immune function of both pro-and anti-inflammation.The results provide insight into the evolution and functional diversification of Bivalvia caspase-8 genes.

关 键 词：CASPASE-8 apoptosis METAMORPHOSIS inflammation Crassostrea gigas 

分 类 号：S917.4[农业科学—水产科学]