糖尿病模型小鼠胰腺组织中死亡受体凋亡通路相关因子的表达  

Expression of death receptor apoptosis pathway-correlated factors in pancreatic tissue of mouse diabetic model

作　　者：苗苗 刘艳[1] 张焕 赵慧超 李卓繁 张浩澜 袁盼盼 MIAO Miao;LIU Yan;ZHANG Huan;ZHAO Huichao;LI Zhuofan;ZHANG Haolan;YUAN Panpan(Department of Emergency,the 83rd Army Group Hospital of the Chinese People's Liberation Army,Xinxiang 453000;College of Animal Science and Veterinary Medicine,Henan Institute of Science and Technology,Xinxiang 453003,China)

机构地区：[1]中国人民解放军陆军第八十三集团军医院急诊科,河南新乡453000 [2]河南科技学院动物科技学院,河南新乡453003

出　　处：《基础医学与临床》2023年第8期1254-1258,共5页Basic and Clinical Medicine

基　　金：新乡市科技攻关项目(GG2020054)。

摘　　要：目的研究糖尿病模型小鼠胰腺组织中死亡受体凋亡通路中相关凋亡因子表达的变化。方法将小鼠分为对照组(control)和模型组(model),2次腹腔注射四氧嘧啶(120 mg/kg,80 mg/kg),在造模后第3和7天,检测血糖;取胰腺组织,苏木精-伊红染色(HE)观察,原位末端标记法(TUNEL)检测凋亡率,RT-qPCR检测肿瘤坏死因子受体1(TNFR1)、Fas相关死亡域(FADD)、半胱氨酸蛋白酶-8(caspase-8)和半胱氨酸蛋白酶-3(caspase-3)的mRNA表达量。结果与对照组比较,模型组小鼠胰腺腺泡细胞出现颗粒变性,毛细血管充血,胰岛体积减小,胰岛内细胞数量减少。与对照组比较,模型组小鼠胰腺内细胞凋亡率明显增多(P<0.01)。另外,与对照组比较,在3和7天,模型组小鼠胰腺组织内Tnfr1、Fadd、caspase-8和caspase-3 mRNA表达均显著或极显著的增加(P<0.05或P<0.01)。结论四氧嘧啶能够促进糖尿病模型小鼠胰腺组织中Tnfr1、Fadd、caspase-8和caspase-3 mRNA的高表达,进而诱导了胰腺细胞的凋亡。Objective To study the expression of apoptosis related factors in the apoptosis pathway of death receptor in the pancreas of mice diabetic models.Methods The mice were divided into control group and model group.They were injected alloxan intraperitoneally twice(120 mg/kg,80 mg/kg).Blood glucose was measured on the third and seventh days after the model was established;Pancreatic tissue was taken for HE observation,TUNEL was used to detect the apoptosis rate,and RT-qPCR was used to detect the mRNA expression of tumor necrosis factor receptor 1(TNFR1),Fas-related death domain(FADD),caspase-8 and caspase-3.Results Compared with the control group,pancreatic acinar cells in the model group showed granular degeneration,capillary congestion,decreased islet volume and the number of cells in islets.Compared with the control group,the apoptosis rate of pancreatic cells in the model group was significantly increased(P<0.01).In addition,compared with the control group,the expression of Tnfr1,Fadd,caspase-8 and caspase-3 mRNA in the pancreas of the model group increased significantly or highly significantly on the third and seventh days(P<0.05 or P<0.01).Conclusions Alloxan could promote the over-expression of Tnfr1,Fadd,caspase-8 and caspase-3 mRNA in pancreatic tissue of diabetes model mice,and then induce apoptosis of pancreatic cells.

关 键 词：四氧嘧啶 胰腺细胞 凋亡 死亡受体凋亡通路 

分 类 号：R587.1[医药卫生—内分泌]