HPLC法测定益真健脑丸中人参皂苷Rg_(1)、Re、Rb_(1)含量  

Determination of ginsenoside Rg_(1),Re and Rb_(1)in Yizhen Jiannao Pills by HPLC

作　　者：谢海龙[1,2] 董晏含 赵波 何嘉郡[1] XIE Hai-long;DONG Yan-han;ZHAO Bo;HE Jia-jun(Heilongjiang University of Chinese Medicine,Harbin 150040,China;Heilongjiang Zhenbaodao Pharmaceutical Co.,Ltd.,Hulin 158400,China)

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040 [2]黑龙江珍宝岛药业股份有限公司,黑龙江虎林158400

出　　处：《化学工程师》2023年第7期27-30,共4页Chemical Engineer

基　　金：黑龙江省自然科学基金联合引导项目(LH2022H087);黑龙江省博士后资助项目(LBH-Z20096);黑龙江中医药大学科研基金项目(2019XY03)。

摘　　要：目的采用HPLC对益真健脑丸中西洋参药材3种主要成分人参皂苷Rg_(1)、Re、Rb_(1)进行含量测定。方法采用C18色谱柱,流动相为乙腈(A)-0.1%磷酸溶液(B),梯度洗脱,检测波长203nm,流速1.0mL·min^(-1),柱温40℃。结果人参皂苷Rg_(1)、Re、Rb_(1)分别在2.00~20.00μg·m L^(-1)、4.00~40.00μg·m L^(-1)、10.00~100.00μg·m L^(-1)浓度范围内线性关系良好。结论该方法简单、稳定,可用于益真健脑丸中3种人参皂苷的含量测定。OBJECTIVE To determine ginsenoside Rg_(1),Re and Rb_(1),the three main components of American ginseng in Yizhen Jiannao Pills by HPLC.METHODS C18 chromatographic column was used.The mobile phase was acetonitrile(A)-0.1%phosphoric acid solution(B),gradient elution.The detection wavelength was 203nm,the flow rate was 1.0 mL·min^(-1),and the column temperature was 40℃.RESULTS Ginsenoside Rg_(1),Re and Rb_(1)had a good linear relationship within the concentration range of 2.00~20.00μg·mL^(-1),4.00~40.00μg·mL^(-1),10.00~100.00μg·mL^(-1)respectively.CONCLUSION The method is simple and stable,and can be used for the determination of three ginsenosides in Yizhen Jiannao Pill.

关 键 词：益真健脑丸 含量测定 人参皂苷 高效液相 西洋参 

分 类 号：R286.0[医药卫生—中药学] O657.72[医药卫生—中医学]