Biosynthesis and transfer of 2‑acetamido‑4‑methylamino‑l‑fucose to the core‑lipid A in Bordetella pertussis  

作　　者：Liangjia Wang Zhen Wang Shuaiwen Wang Xiaoqing Hu Xiaoyuan Wang 

机构地区：[1]State Key Laboratory of Food Science and Technology,Jiangnan University,1800 Lihu Avenue,Wuxi 214122,China [2]International Joint Laboratory on Food Safety,Jiangnan University,Wuxi 214122,China [3]Key Laboratory of Industrial Biotechnology,Ministry of Education,School of Biotechnology,Jiangnan University,Wuxi 214122,China

出　　处：《Systems Microbiology and Biomanufacturing》2023年第2期273-286,共14页系统微生物学与生物制造（英文）

基　　金：supported by the National Key Research and Development Program of China(2018YFA0900300);the Postgraduate Research&Practice Innovation Program of Jiangsu Province(CX10295).

摘　　要：The trisaccharide unit in lipopolysaccharide of Bordetella pertussis is important for immune recognition,but how the rare sugar 2-acetamido-4-methylamino-fucose in the trisaccharide unit is synthesized and transferred to the core of lipopolysaccharide in B.pertussis remains unclear.In this study,we demonstrated that the genes bplF,bplG,bplL and bplI are involved in the synthesis and transfer of 2-acetamido-4-methylamino-fucose to the core of lipopolysaccharide in B.pertussis.These genes were overexpressed with various combination in a recombinant E.coli strain which can synthesize B.pertussis corelipid A.Lipopolysaccharides were isolated from these strains and analyzed by using SDS-PAGE,ion chromatography and NMR.Based on these analyses,the mechanism of biosynthesis and transfer of 2-acetamido-4-methylamino-l-fucose to the core-lipid A in B.pertussis is proposed.

关 键 词：Bordetella pertussis Escherichia coli LIPOPOLYSACCHARIDE Trisaccharide unit 2-Acetamido-4-methylamino-L-fucose BplL 

分 类 号：Q93[生物学—微生物学]