CGD-1, a defensin-related peptide derived from marine Chinese medicine Ostreae concha, inhibits the Gram-negative bacteria by membrane attack  

作　　者：Wei-Tao Wang Qing-Zhou Meng Long-Fen Li Wei-Yi Meng Xin Yin Pei-Pei Zhao Meng-Meng Wang Jing-Li Ren Xue-Kui Xia Chun-Lei Li 

机构地区：[1]Department of Pharmacy,Qilu Hospital of Shandong University,Jinan 250001,China [2]Biology Institute,Qilu University of Technology(Shandong Academy of Sciences),Jinan 250021,China

出　　处：《TMR Pharmacology Research》2023年第2期38-45,共8页TMR药理学研究

基　　金：This research work was financially supported National Natural Science Foundation of China(No.82003910);Guangdong Basic and Applied Basic Research Foundation(2020A1515110550);Funding Key R&D Program of Shandong Province(2022SFGC0105);Exploration Innovation Team(2021GXRC062),Jinan Talent Project for Universities(202228088);Key innovation Project of Qilu University of Technology(Shandong Academy of Sciences)(2022JBZ01-06).

摘　　要：There is an increasing interest in discovering new antibacterial agents derived from nature to enhance the treatment of various bacterial infections.Defensins and their derived peptide fragments exhibit significant antibacterial activity without any cytotoxic effects,making them attractive features for potential novel antibacterial therapeutics.Crassostrea gigas,a traditional seafood that has been used worldwide for centuries,has its shells applied in Chinese medicine as Ostreae concha.In this study,bioinformatics analysis was used to obtain a novel antibacterial peptide,CGD-1,derived from marine Chinese medicine Ostreae concha.The physicochemical characterization and circular dichroism analysis results demonstrated that CGD-1 assembled into anα-helical structure in a simulated membrane environment,and it displayed antibacterial action against Gram-negative bacteria.The minimal inhibitory concentrations against both Pseudomonas aeruginosa ATCC27853 and Escherichia coli ATCC25922 were 25μM.CGD-1 was able to efficiently permeate the cell membrane.Changes in bacterial cell morphology were evaluated using a field emission scanning electron microscope.The results suggested that CGD-1 exerted its antibacterial activity through permeabilizing and disrupting the bacterial cell membrane.Therefore,CGD-1 may have potential applications in fighting against pathogenic bacteria such as P.aeruginosa and E.coli.

关 键 词：DEFENSIN antibacterial activity HEMOLYMPH Crassostrea gigas Ostreae concha 

分 类 号：O62[理学—有机化学]