Boosting wheat functional genomics via an indexed EMS mutant library of KN9204  

作　　者：Dongzhi Wang Yongpeng Li Haojie Wang Yongxin Xu Yiman Yang Yuxin Zhou Zhongxu Chen Yuqing Zhou Lixuan Gui Yi Guo Chunjiang Zhou Wenqiang Tang Shuzhi Zheng Lei Wang Xiulin Guo Yingjun Zhang Fa Cui Xuelei Lin Yuling Jiao Yuehui He Junming Li Fei He Xigang Liu Jun Xiao 

机构地区：[1]Key Laboratory of Plant Cell and Chromosome Engineering,Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Beijing 100101,China [2]Ministry of Education Key Laboratory of Molecular and Cellular Biology,Hebei Key Laboratory of Molecular and Cellular Biology,College of Life Sciences,Hebei Normal University,Hebei Collaboration Innovation Center for Cell Signaling,Shijiazhuang 050024,China [3]Center for Agricultural Resources Research,Institute of Genetics and Development Biology,Chinese Academy of Sciences,Shijiazhuang 050022,China [4]University of Chinese Academy of Sciences,Beijing 100049,China [5]Department of Life Science,Tcuni,Inc,Chengdu 610000,China [6]State Key Laboratory of Protein and Plant Gene Research,Peking-Tsinghua Center for Life Sciences,School of Life Sciences,Center for Quantitative Biology,Peking University,Beijing 100871,China [7]State Key Laboratory of Protein and Plant Gene Research,School of Advanced Agricultural Sciences,Peking-Tsinghua Center for Life Sciences,Peking University,Beijing 100871,China [8]Plant Genetic Engineering Center of Hebei Province,Institute of Biotechnology and Food Science,Hebei Academy of Agriculture and Forestry Sciences,Shijiazhuang 050051,China [9]Institute ofCereal and OilCrops,Hebei Academy of Agriculture and Forestry Sciences,Laboratory of CropGenetics andBreeding ofHebei,Shijiazhuang 050035,China [10]Key Laboratory of Molecular Module-Based Breeding of High Yield and Abiotic Resistant Plants in Universities of Shandong,College of Agriculture,Ludong University,Yantai 264025,China [11]Centre of Excellence for Plant and Microbial Science(CEPAMS),JIC-CAS,Beijing 100101,China

出　　处：《Plant Communications》2023年第4期58-76,共19页植物通讯（英文）

基　　金：supported by the Strategic Priority Research Program of the Chinese Academy of Sciences(XDA24010204)to J.X.,the Hebei Natural Science Foundation(C2021205013);"Full-time introduction of high-end talent research project"(2020HBQZYC004)to X.-g.L.;the National Natural Science Foundation of China(U22A6009)to J.-m.L.;the Research Program for Network Security and Information of the Chinese Academy of Sciences(CAS-WX2021SF-0109)to F.H.and J.X.;the National Key Research and Developmental Program of China(2021YFD1201500)to J.X.;a China Postdoctoral Science Foundation-funded project(2020M680742)to D.-z.W.

摘　　要：A better understanding of wheat functional genomics can improve targeted breeding for better agronomic traits and environmental adaptation.However,the lack of gene-indexed mutants and the low transformation efficiency of wheat limit in-depth gene functional studies and genetic manipulation for breeding.In this study,we created a library for KN9204,a popular wheat variety in northern China,with a reference genome,transcriptome,and epigenome of different tissues,using ethyl methyl sulfonate(EMS)mutagenesis.This library contains a vast developmental diversity of critical tissues and transition stages.Exome capture sequencing of 2090 mutant lines using KN9204 genome-designed probes revealed that 98.79%of coding genes had mutations,and each line had an average of 1383 EMS-type SNPs.We identified new allelic variations for crucial agronomic trait-related genes such as Rht-D1,Q,TaTB1,and WFZP.We tested 100 lines with severemutations in 80 NAC transcription factors(TFs)under drought and salinity stress and identified 13 lines with altered sensitivity.Further analysis of three lines using transcriptome and chromatin accessibility data revealed hundreds of direct NAC targets with altered transcription patterns under salt or drought stress,including SNAC1,DREB2B,CML16,and ZFP182,factors known to respond to abiotic stress.Thus,we have generated and indexed a KN9204 EMS mutant library that can facilitate functional genomics research and offer resources for genetic manipulation of wheat.

关 键 词：WHEAT exome capture sequencing EMS mutagenesis functional genomics 

分 类 号：S512.1[农业科学—作物学]