可注射导电水凝胶聚吡咯-壳聚糖对慢性心肌梗死后心脏功能长期改善  

Long⁃term improvement of cardiac function after myocardial infarction with infectable Polypyrrole⁃Chito⁃san

作　　者：王琦 王馨竹 姜增誉[2] 周景莉 何生[2] WANG Qi;WANG Xinzhu;JIANG Zengyu;ZHOU Jingli;HE Sheng(School of Basic Medical Sciences,Shanxi Medical University,Taiyuan 030001,China;不详)

机构地区：[1]山西医科大学基础医学院,太原030001 [2]山西医科大学第一医院影像科,太原030001 [3]山西医科大学附属人民医院心内科,太原030001

出　　处：《实用医学杂志》2023年第13期1606-1613,共8页The Journal of Practical Medicine

基　　金：国家自然科学基金项目(编号:81900274);山西省自然科学基金项目(编号:202103021223426)。

摘　　要：目的探讨可注射导电水凝胶聚吡咯-壳聚糖(Polypyrrole⁃Chitosan,PPY⁃CHI)对慢性心肌梗死后心脏功能长期改善的有效性及机制。方法通过将聚吡咯嫁接到壳聚糖(Chitosan,CHI)骨架上,合成导电复合物PPY⁃CHI。在体内,采用大鼠慢性心肌梗死模型,将梗后SD雌性大鼠随机分为3组(PPY⁃CHI、CHI及Saline组,n=5)。4周后,分别在心肌梗死区及周边植入PPY⁃CHI。用超声、马松染色、免疫荧光和心肌电阻率评估心功能长期改善情况。结果移植生物材料22周后,体内评估发现,与注射CHI和Saline相比,心肌内注射PPY⁃CHI具有更低的组织电阻,毛细血管密度大大增加,瘢痕区心室壁厚度显著增加,LVFS较梗后增加(10.6±0.55)%。结论心肌内注射PPY⁃CHI促进血管生成,降低瘢痕区组织电阻和抑制心室重塑,加强慢性心肌梗死后心功能的恢复。Objective To investigate the efficacy and mechanism of injectable conductive hydrogel Polypyrrole⁃Chitosan(PPY⁃CHI)on long⁃term improvement of cardiac function after chronic myocardial infarction.Methods PPY⁃CHI,a conductive complex,was synthesized by coupling Polypyrrole to a Chitosan(CHI)back⁃bone.A rat chronic myocardial infarction model was constructed in vivo,post⁃infarction SD female rats were ran⁃domly divided into three groups(PPY⁃CHI,CHI and Saline groups,n=5).PPY⁃CHI was implanted in and around the infarct area four weeks later.Long⁃term improvement in cardiac function was assessed by ultrasound,Masson staining,immunofluorescence and myocardial resistivity.Results After 22 weeks of transplantation of the bioma⁃terials,in vitro evaluation revealed that intracardiac injection of PPY⁃CHI had lower tissue resistance,greatly increased capillary density,significantly increased ventricular wall thickness in the scar area,and a(10.6±0.55)%increase in LVFS compared to post⁃infarction compared to CHI and Saline injections.Conclusions Intracardiac injection of PPY⁃CHI promoted angiogenesis,reduced tissue resistance in the scar region,and inhibited adverse ventricular remodeling,which enhancethe recovery of cardiac function after chronic infarction.

关 键 词：可注射导电水凝胶 慢性心肌梗死 心脏组织工程 生物材料 

分 类 号：R542.2[医药卫生—心血管疾病]