对羟基苯甲酸对三七黑斑病致病性因素的影响研究  

Study on Effects of p-hydroxybenzoic Acid on the Pathogenic Factors of Black Spot Disease in Panax notoginseng

作　　者：胡光玉 杨善武 周彬 赵春琼 陈健好 赵静 HU Guang-yu;YANG Shan-wu;ZHOU Bin(College of Chemistry,Biology and Environment,Yuxi Normol University,Yuxi,Yunnan 653100)

机构地区：[1]玉溪师范学院化学生物与环境学院,云南玉溪653100

出　　处：《安徽农业科学》2023年第13期163-165,171,共4页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金项目(32060036);大学生创新创业项目(202111390001)。

摘　　要：[目的]探讨对羟基苯甲酸与三七黑斑病“病害三角”之间的互作影响,分析对羟基苯甲酸对病原真菌及其寄主植物在生理功能、抗病性和病原菌致病性上的影响。[方法]以三七一年生幼苗及三七黑斑病菌(Altemaria panax Whetzel)为试材,借助酶学分析技术,分别探讨4个不同浓度对羟基苯甲酸胁迫下三七-土壤-病原菌致病性生态因素的变化。[结果]不同浓度的对羟基苯甲酸对三七幼苗及病原菌的化感作用受到浓度效应的影响。在2.5~5.0 mmol/L浓度下,三七根系抗性酶活性会有短期内的增加,后逐渐降低,而高浓度(10.0 mmol/L)的胁迫下则明显下降;土壤酶活性的变化也有相同的趋势。三七黑斑病菌在2.5~5.0 mmol/L浓度下其果胶酶、纤维素酶均出现较高活性,说明在该浓度范围内病原菌生长情况及繁殖能力较好。[结论]对羟基苯甲酸与三七黑斑病发生的3个要素即寄主、土壤、病原菌三者之间存在化感浓度效应。[Objective]To explore the interaction between p-hydroxybenzoic acid and the“disease triangle”of Panax notoginseng black spot disease,and analyze the effects of p-hydroxybenzoic acid on the physiological function,disease resistance and pathogenicity of pathogenic fungi and their host plants.[Method]Using one-year seedlings of Panax notoginseng and Alteraria panax Whetzel as test materials,the enzymatic analysis techniques were used to investigate the changes in the pathogenic ecological factors of Panax notoginseng-soil-pathogen under four different concentrations of p-hydroxybenzoic acid stress.[Result]The allelopathy of different concentrations of p-hydroxybenzoic acid on the seedlings and pathogens of Panax notoginseng was affected by the concentration effect.At a concentration of 2.5-5.0 mmol/L,the root resistance enzyme activity of Panax notoginseng increased in a short period of time,and then gradually decreased,while under high concentration(10.0 mmol/L),it decreased significantly;the changes of soil enzyme activity showed the same trend.At the concentration of 2.5-5.0 mmol/L,the pectinase and cellulase activity of Panax notoginseng were higher,which indicated that the growth and reproduction ability of the pathogenic bacteria were relatively good within this concentration range.[Conclusion]There is an allelopathic concentration effect of p-hydroxybenzoic acid on the three factors involved in the occurrence of black spot disease of Panax notoginseng,namely host,soil and pathogenic bacteria.

关 键 词：对羟基苯甲酸 三七黑斑病 病原真菌 抗性酶 土壤酶活性 致病性酶 

分 类 号：S432.1[农业科学—植物病理学]