玉米叶宽的QTL定位及全基因组选择分析  

QTL mapping and genomic selection of maize leaf width

作　　者：陈占辉 孙强 任姣姣 黄博文 许加波 杨杰[2] 吴鹏昊[1] CHEN Zhanhui;SUN Qiang;REN Jiaojiao;HUANG Bowen;XU Jiabo;YANG Jie;WU Penghao(College of Agronomy,Xinjiang Agricultural University,Urumqi 830052,China;Institute of Food Crops,Xinjiang Academy of Agricultural Sciences,Urumqi 830091,China)

机构地区：[1]新疆农业大学农学院,乌鲁木齐830052 [2]新疆农业科学院粮食作物研究所,乌鲁木齐830052

出　　处：《新疆农业科学》2023年第7期1606-1613,共8页Xinjiang Agricultural Sciences

基　　金：国家自然科学基金项目“玉米生物诱导单倍体母本被诱导关键性状的遗传解析和全基因组选择”(U2003304);国家自然科学基金“玉米田间耐旱性遗传解析及全基因组预测研究”(32001561);国家自然科学基金“玉米单倍体雌穗自然加倍遗传解析及全基因组预测研究”(32060484);自治区天山创新团队“玉米生物育种及配套技术创新团队”(2022D14017);自治区自然科学基金重点基金“基于机器学习多组学技术对玉米大田耐旱的分子基础遗传解析与功能基因挖掘”(2022D01D34);自治区重点研发项目“玉米绿色丰产提质增效技术优化集成及应用-伊犁河谷区玉米绿色高效种植技术研究与示范”(2021B02002-2-2)。

摘　　要：【目的】分析控制玉米叶宽的关键QTL位点,为选育具有理想株型的玉米奠定基础。【方法】以玉米自交系B73和郑58为亲本构建F 2∶3家系,采用液相48k探针捕获技术检测基因型,对多环境下玉米叶宽表型进行QTL定位和全基因组选择。【结果】叶宽在基因型、环境、基因型与环境的互作变异项都具有显著差异,遗传力为0.39。共检测到12个穗位叶宽相关QTL位点,分别位于第1、3、4、5、8和10号染色体,表型贡献率为3.75%~16.17%。位于bin 1.06和bin 5.01的2个QTL在多环境下被检测到,具有环境稳定性,其中位于bin 5.01的QTL为主效位点,可用于精细定位研究。当SNP标记个数为300、训练群体占总群体50%时即可得到较好的预测精度。【结论】玉米叶宽是由主效多基因控制的,全基因组选择可以加速玉米叶宽性状的选育效率。【Objective】To carry out ggenetic analysis of the leaf width of maize and detection of major QTL controlling leaf in the hope of providing an important theoretical basis for breeding maize with ideal plant type.【Methods】In this experiment,the F 2∶3 family was constructed from the maize inbred lines B73 and Zheng 58 which were genotyped by liquid phase 48k probe capture technology and phenotyped in multi-environment trails for QTL mapping and genomic selection.【Results】The results showed that the leaf width displayed significant differences in genotype,environment,and genotype and environment interaction.The broad-sense heritability was 0.39.A total of 12 QTL distributed on chromosomes 1,3,4,5,8 and 10 were identified for leaf width.The phenotypic variation explained of each QTL ranged from 3.75%to 16.17%.Two loci,located on bin1.06 and bin5.01,were stable QTL detected in multiple environments.The QTL located on bin 5.01 was a major QTL,which could be used for fine mapping.When the number of SNPs reached 300 and the training population size reached 50%.【Conclusion】Leaf width is controlled by major polygenes,and genome selection can accelerate the efficiency of leaf wide traits.

关 键 词：玉米 叶宽 数量性状位点 全基因组选择 

分 类 号：S513[农业科学—作物学]