不同谷子品种萌发期抗旱鉴定及生理变化  

Drought resistance identification and physiological changes of different millet varieties during germination

作　　者：柏玲 冯国郡[2] 胡相伟[2] 赵云 石书兵[1] BAI Ling;FENG Guojun;HU Xiangwei;ZHAO Yun;SHI Shubing(College of Agriculture,Xinjiang Agricultural University,Urumqi 830052,China;Institute of Food Crops,Xinjiang Academy of Agricultural Sciences,Urumqi 830091,China)

机构地区：[1]新疆农业大学农学院,乌鲁木齐830052 [2]新疆农业科学院粮食作物研究所,乌鲁木齐830091

出　　处：《新疆农业科学》2023年第7期1630-1640,共11页Xinjiang Agricultural Sciences

基　　金：国家自然科学基金项目“谷子种质资源抗旱性精准鉴定及耐旱相关基因的挖掘”(3206150401);国家谷子高粱产业技术体系乌鲁木齐综合试验站(CARS-06-14.5-B30)。

摘　　要：【目的】研究出谷子萌发期抗旱性相关指标,分析不同谷子品种萌发期内在抗氧化酶对干旱胁迫的响应机理,为筛选和培育适宜新疆地区种植的谷子品种提供参考。【方法】用20%的聚乙二醇(PEG-6000)模拟萌发期干旱胁迫对15个谷子品种进行抗旱性鉴定,测定15个品种在干旱胁迫条件下SOD、POD、CAT和GR的活性。【结果】(1)干旱胁迫下,受试品种谷子的发芽势、发芽率、胚根长、胚芽长、芽鲜重、芽干重均受到不同程度的抑制,对发芽率的影响相对较小,但蒙丰谷7号等几个品种的根芽具有促进作用。(2)用隶属函数法进行抗旱性综合评价,将15个谷子品种的抗旱性划分等级,其中强抗旱型品种5个、中度抗旱型品种4个、干旱敏感型品种5个、干旱极敏感型1个。(3)干旱胁迫下不同品种谷子幼苗抗氧化酶SOD、POD、CAT、GR活性均有所上升,且耐旱性品种本身抗氧化酶活性就比较高且在干旱胁迫下维持较高水平。【结论】蒙丰谷7号的抗旱性最好。【Objective】To explore the impact of drought on millet germination,screen out drought resistance related indicators during millet germination,and further clarify the response mechanism of antioxidant enzymes in different millet varieties to drought stress during germination.the research results can provide a reference for selecting and cultivating millet varieties suitable for planting in Xinjiang.【Methods】Using 20%polyethylene glycol(PEG-6000)to simulate drought stress during germination,the drought resistance of 15 millet varieties was evaluated,and the changes in the activities of SOD,POD,CAT,and GR of 15 varieties under drought stress were measured to study their internal situation.【Results】(1)Under drought stress,the germination potential,germination rate,radicle length,plumule length,fresh weight,and dry weight of the tested millet varieties were inhibited to varying degrees,with a relatively small impact on the germination rate,but the root buds of several varieties such as Mengfenggu 7 had a promoting effect.(2)A comprehensive evaluation of drought resistance was conducted using the membership function method.The drought resistance of 15 millet varieties was graded,including 5 strong drought resistant varieties,4 moderate drought resistant varieties,5 drought sensitive varieties,and 1 drought extremely sensitive type.(3)Under drought stress,the activities of antioxidant enzymes SOD,POD,CAT,and GR in millet seedlings of different varieties increased,and the drought tolerant varieties themselves had relatively high antioxidant enzyme activities and could maintain high levels under drought stress.【Conclusion】Overall,Mengfenggu 7 has the best drought resistancehe.

关 键 词：谷子 PEG 抗氧化酶 隶属函数值 抗旱鉴定 

分 类 号：S515[农业科学—作物学]