柽柳根际土壤放线菌的分离及拮抗活性筛选  

Isolation and antagonistic activity screening of Actinomycetes from rhizosphere soil of Tamarix

作　　者：陈乙煌 邢利 东珍珍 马小梅 黄建军 罗晓霞[1] CHEN Yihuang;XING Li;DONG Zhenzhen;MA Xiaomei;HUANG Jianjun;LUO Xiaoxia(College of Life Sciences,Tarim University,Aral Xinjiang 843300,China;Aral Municipal Agricultural Development Service Center of 13th Agricultural Regiment,Aral Xinjiang 843300,China)

机构地区：[1]塔里木大学生命科学学院,新疆阿拉尔843300 [2]新疆阿拉尔市十三团农业发展服务中心,新疆阿拉尔843300

出　　处：《新疆农业科学》2023年第7期1790-1797,共8页Xinjiang Agricultural Sciences

基　　金：新疆生产建设兵团中青年领军人才项目(2019CB030);区域创新引导计划(2021BB007)。

摘　　要：【目的】分析柽柳根际土壤放线菌物种多样性,研究其抗菌活性,为新疆主要农牧业病害的防控奠定菌种基础。【方法】选择新疆南疆地区10种柽柳根际土壤的放线菌资源,采用四种培养基,通过菌株16SrDNA测序,采用平板对峙法筛选抗菌活性。【结果】分离出294株菌株,鉴定的150株,分属于7个纲12个目16科24个属,另有9个潜在新种,以金黄色葡萄球菌、铜绿假单胞菌、大肠杆菌、肺炎克雷伯菌、解淀粉欧文氏菌、伤寒沙门氏菌、白色念珠菌、链格孢菌、棉花黄萎病菌、棉花枯萎病菌为靶标菌。【结论】筛选出68株活性放线菌。柽柳根际土壤放线菌的分离丰富了微生物资源及菌种的储备。【Objective】To lay a foundation for the prevention and control of major agricultural and animal husbandry diseases in Xinjiang.【Methods】Ten species of actinomycetes from rhizosphere soils of Tamarix ramosissima in southern Xinjiang were selected and their antibactieral activities were studied.Species diversity of actinomycetes in rhizosphere soil of Tamarix ramosissima was analyzed by 16SrDNA sequencing using four kinds of media.The antibacterial activity was screened by plate confrontation methods.The antibacterial activity was screened by plate confrontation method.【Results】The results showed that total 294 strains were isolated,and total 150 strains were identified,which belonged to seven classes and twelve orders,total 12 families,total 24 genera,and 9 potential new species.At the same time,the antibacterial activity was analyzed with Staphylococcus aureus,Pseudonas aeruginosa,Escherichia coli,Klebsiella pneumonia,Erwinia amylohydrolytic,Slmonella typhoid,Candida albicans,Chain bacteria Verticilliuma dhiiae,Fusarium oxysporum as the target bacteria.【Conclusion】Total 68 strains of active actinmycetes were screened by antibacterial activity analysis.Isolation of actinomycetes from rhizosphere soil of Tamarix ramosissima enriched microbial resources and species reserve.

关 键 词：柽柳根际土壤 放线菌 物种多样性 抗菌活性 

分 类 号：Q939.96[生物学—微生物学] S482.7[农业科学—农药学]