CRISPR/Cas systems for the detection of nucleic acid and nonnucleic acid targets  

作　　者：Weiran Su Junru Li Chen Ji Congshuo Chen Yuzheng Wang Huili Dai Fengqin Li Peifeng Liu 

机构地区：[1]State Key Laboratory of Oncogenes and Related Genes,Shanghai Cancer Institute,Renji Hospital,School of Medicine,Shanghai Jiao Tong University,Shanghai 200032,China [2]Central Laboratory,Renji Hospital,School of Medicine,Shanghai Jiao Tong University,Shanghai 200127,China [3]Micro-Nano Research and Diagnosis Center,Renji Hospital,School of Medicine,Shanghai Jiao Tong University,Shanghai 200127,China

出　　处：《Nano Research》2023年第7期9940-9953,共14页纳米研究（英文版）

基　　金：This work was financially supported by the National Natural Science Foundation of China(Nos.81771968 and 22204104);the Shanghai Sailing Program(No.21YF1444900);the Shanghai Municipal Natural Science Foundation(No.22ZR1459600);the Medical-Engineering Joint Funds from the Shanghai Jiao Tong University(Nos.YG2023ZD07 and YG2021QN23);the Foundation of Shanghai Municipal Health Commission(No.2022JC002);the Innovative Research Team of High-Level Local Universities in Shanghai,China.

摘　　要：Clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated(Cas)systems are becoming powerful tools for disease biomarkers detection.Due to the specific recognition,cis-cleavage and nonspecific trans-cleavage capabilities,CRISPR/Cas systems have implemented the detection of nucleic acid targets(DNA and RNA)as well as non-nucleic acid targets(e.g.,proteins,exosomes,cells,and small molecules).In this review,we first summarize the principles and characteristics of various CRISPR/Cas systems,including CRISPR/Cas9,Cas12,Cas13 and Cas14 systems.Then,various types of applications of CRISPR/Cas systems used in detecting nucleic and non-nucleic acid targets are introduced emphatically.Finally,the prospects and challenges of their applications in biosensing are discussed.

关 键 词：clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated(Cas) DETECTION nucleic acid targets non-nucleic acid targets 

分 类 号：Q78[生物学—分子生物学]